Genomic expression of mesenchymal stem cells to altered nanoscale topographies
- 12 February 2008
- journal article
- research article
- Published by The Royal Society in Journal of The Royal Society Interface
- Vol. 5 (26), 1055-1065
- https://doi.org/10.1098/rsif.2008.0016
Abstract
The understanding of cellular response to the shape of their environment would be of benefit in the development of artificial extracellular environments for potential use in the production of biomimetic surfaces. Specifically, the understanding of how cues from the extracellular environment can be used to understand stem cell differentiation would be of special interest in regenerative medicine. In this paper, the genetic profile of mesenchymal stem cells cultured on two osteogenic nanoscale topographies (pitted surface versus raised islands) are compared with cells treated with dexamethasone, a corticosteroid routinely used to stimulate bone formation in culture from mesenchymal stem cells, using 19k gene microarrays as well as 101 gene arrays specific for osteoblast and endothelial biology. The current studies show that by altering the shape of the matrix a cell response (genomic profile) similar to that achieved with chemical stimulation can be elicited. Here, we show that bone formation can be achieved with efficiency similar to that of dexamethasone with the added benefit that endothelial cell development is not inhibited. We further show that the mechanism of action of the topographies and dexamethasone differs. This could have an implication for tissue engineering in which a simultaneous, targeted, development of a tissue, such as bone, without the suppression of angiogenesis to supply nutrients to the new tissue is required. The results further demonstrate that perhaps the shape of the extracellular matrix is critical to tissue development.Keywords
This publication has 52 references indexed in Scilit:
- Expression of cytokines IL-4, IL-12, IL-15, IL-18, and IFNγ and modulation by different growth factors in cultured human osteoblast-like cellsJournal of Bone and Mineral Metabolism, 2007
- Nanomechanotransduction and Interphase Nuclear Organization influence on genomic controlJournal of Cellular Biochemistry, 2007
- Nanotopographical stimulation of mechanotransduction and changes in interphase centromere positioningJournal of Cellular Biochemistry, 2006
- Changes in fibroblast morphology in response to nano-columns produced by colloidal lithographyBiomaterials, 2004
- Rank products: a simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experimentsFEBS Letters, 2004
- Cell Shape, Cytoskeletal Tension, and RhoA Regulate Stem Cell Lineage CommitmentDevelopmental Cell, 2004
- Fibroblast response to a controlled nanoenvironment produced by colloidal lithographyJournal of Biomedical Materials Research Part A, 2004
- Induction of Human Osteoprogenitor Chemotaxis, Proliferation, Differentiation, and Bone Formation by Osteoblast Stimulating Factor-1/Pleiotrophin: Osteoconductive Biomimetic Scaffolds for Tissue EngineeringJournal of Bone and Mineral Research, 2003
- Compression-induced changes in the shape and volume of the chondrocyte nucleusJournal of Biomechanics, 1995
- Nuclear matrix and the regulation of gene expression: Tissue specificityJournal of Cellular Biochemistry, 1994