Antimicrobial activity in cultures of endophytic fungi isolated fromGarciniaspecies
Open Access
- 1 December 2006
- journal article
- Published by Oxford University Press (OUP) in FEMS Immunology & Medical Microbiology
- Vol. 48 (3), 367-372
- https://doi.org/10.1111/j.1574-695x.2006.00155.x
Abstract
The aim of the present study was to screen for antimicrobial activity in endophytic fungi isolated from surface sterilized leaves and branches of five Garcinia plants, G. atroviridis, G. dulcis, G. mangostana, G. nigrolineata and G. scortechinii, found in southern Thailand. Fermentation broths from 377 isolated fungi were tested for antimicrobial activity by the agar diffusion method. Minimum inhibitory concentrations (MICs) were obtained for crude ethyl acetate extracts. Seventy isolates (18.6%) displayed antimicrobial activity against at least one pathogenic microorganism, such as Staphylococcus aureus, a clinical isolate of methicillin-resistant S. aureus, Candida albicans and Cryptococcus neoformans. The results revealed that 6–10%, 1–2% and 18% of the crude ethyl acetate extracts inhibited both strains of S. aureus (MIC 32–512 µg mL−1), Ca. albicans and Cr. neoformans (MIC 64–200 µg mL−1), and Microsporum gypseum (MIC 2–64 µg mL−1), respectively. Isolates D15 and M76 displayed the strongest antibacterial activity against both strains of S. aureus. Isolates M76 and N24 displayed strong antifungal activity against M. gypseum. Fungal molecular identification based on internal transcribed spacer rRNA gene sequence analysis demonstrated that isolates D15 (DQ480353), M76 (DQ480360) and N24 (DQ480361) represented Phomopsis sp., Botryosphaeria sp. and an unidentified fungal endophyte, respectively. These results indicate that some endophytic fungi from Garcinia plants are a potential source of antimicrobial agents.Keywords
This publication has 12 references indexed in Scilit:
- Pimarane Diterpene and Cytochalasin Derivatives from the Endophytic Fungus Eutypella scoparia PSU-D44Journal of Natural Products, 2006
- 3-Nitropropionic Acid (3-NPA), a Potent Antimycobacterial Agent from Endophytic Fungi: Is 3-NPA in Some Plants Produced by Endophytes?Journal of Natural Products, 2005
- Anti-Helicobacter pylori substances from endophytic fungal culturesWorld Journal of Microbiology and Biotechnology, 2005
- New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungusMicrobiology, 2004
- Aspergillus fumigatus CY018, an endophytic fungus in Cynodon dactylon as a versatile producer of new and bioactive metabolitesJournal of Biotechnology, 2004
- Endophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plantsWorld Journal of Microbiology and Biotechnology, 2004
- Endophytes as sources of bioactive productsMicrobes and Infection, 2003
- Endophytic fungi: a source of novel biologically active secondary metabolitesMycological Research, 2002
- Characteristics and antifungal activity of a chitin binding protein from Ginkgo bilobaFEBS Letters, 2000
- Basic local alignment search toolJournal of Molecular Biology, 1990