Acetylation of GATA-1 is required for chromatin occupancy
- 1 December 2006
- journal article
- Published by American Society of Hematology in Blood
- Vol. 108 (12), 3736-3738
- https://doi.org/10.1182/blood-2006-07-032847
Abstract
All 3 hematopoietic GATA transcription factors, GATA-1, GATA-2, and GATA-3, are acetylated, although the in vivo role of this modification remains unclear. We examined the functions of an acetylation-defective mutant of GATA-1 in maturing erythroid cells. We found that removal of the acetylation sites in GATA-1 does not impair its nuclear localization, steady-state protein levels, or its ability to bind naked GATA elements in vitro. However, chromatin immunoprecipitation (ChIP) experiments revealed that mutant GATA-1 was dramatically impaired in binding to all examined cellular target sites in vivo, including genes that are normally activated and repressed by GATA-1. Together, these results suggest that acetylation regulates chromatin occupancy of GATA-1. These findings point to a novel function for transcription factor acetylation, perhaps by facilitating protein interactions required for stable association with chromatin templates in vivo.Keywords
This publication has 17 references indexed in Scilit:
- GATA-1: One protein, many partnersThe International Journal of Biochemistry & Cell Biology, 2005
- GATA1 Function, a Paradigm for Transcription Factors in HematopoiesisMolecular and Cellular Biology, 2005
- GATA1 in normal and malignant hematopoiesisSeminars in Cell & Developmental Biology, 2004
- Coregulator-dependent facilitation of chromatin occupancy by GATA-1Proceedings of the National Academy of Sciences, 2004
- Context-dependent regulation of GATA-1 by friend of GATA-1Proceedings of the National Academy of Sciences of the United States of America, 2003
- Functional regulation of GATA-2 by acetylationJournal of Leukocyte Biology, 2003
- Self-Association of Gata1 Enhances Transcriptional Activity In Vivo in Zebra Fish EmbryosMolecular and Cellular Biology, 2003
- Determinants of GATA-1 Binding to DNAPublished by Elsevier BV ,2003
- Formation of a Tissue-Specific Histone Acetylation Pattern by the Hematopoietic Transcription Factor GATA-1Molecular and Cellular Biology, 2003
- NMR Structure of a Specific DNA Complex of Zn-Containing DNA Binding Domain of GATA-1Science, 1993