Cellular and Matrix Mechanics of Bioartificial Tissues During Continuous Cyclic Stretch
Open Access
- 11 October 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC in Annals of Biomedical Engineering
- Vol. 34 (11), 1678-1690
- https://doi.org/10.1007/s10439-006-9153-1
Abstract
Bioartificial tissues are useful model systems for studying cell and extra-cellular matrix mechanics. These tissues provide a 3D environment for cells and allow tissue components to be easily modified and quantified. In this study, we fabricated bioartificial tissue rings from a 1 ml solution containing one million cardiac fibroblasts and 1 mg collagen. After 8 days, rings compacted to <1% of original volume and cell number increased 2.4 fold. We initiated continuous cyclic stretching of the rings after 2, 4, or 8 days of incubation, while monitoring the tissue forces. Peak tissue force during each cycle decreased rapidly after initiating stretch, followed by further slow decline. We added 2 μM Cytochalasin-D to some rings prior to initiation of stretch to determine the force contributed by the matrix. Cell force was estimated by subtracting matrix force from tissue force. After 12 h, matrix force-strain curves were highly nonlinear. Cell force-strain curves were linear during loading and showed hysteresis indicating viscoelastic behavior. Cell stiffness increased with stretching frequency from 0.001–0.25 Hz. Cell stiffness decreased with stretch amplitude (5–25%) at 0.1 Hz. The trends in cell stiffness do not fit simple viscoelastic models previously proposed, and suggest possible strain-amplitude related changes during cyclic stretch.Keywords
This publication has 20 references indexed in Scilit:
- Tissue Cells Feel and Respond to the Stiffness of Their SubstrateScience, 2005
- Adaptation to mechanical load determines shape and properties of heart and circulation: the CircAdapt modelAmerican Journal of Physiology-Heart and Circulatory Physiology, 2005
- Biaxial failure properties of planar living tissue equivalentsJournal of Biomedical Materials Research Part A, 2005
- What makes vessels grow with exercise training?Journal of Applied Physiology, 2004
- Properties of engineered vascular constructs made from collagen, fibrin, and collagen–fibrin mixturesBiomaterials, 2004
- Regulation of mechanical interactions between fibroblasts and the substratum by stretch-activated Ca2+ entryJournal of Cell Science, 2004
- Design and Application of a Test System for Viscoelastic Characterization of Collagen GelsTissue Engineering, 2004
- Mechanical Strain-Stimulated Remodeling of Tissue-Engineered Blood Vessel ConstructsTissue Engineering, 2003
- Influence of External Uniaxial Cyclic Strain on Oriented Fibroblast-Seeded Collagen GelsTissue Engineering, 2003
- Regulation of LPA-Promoted Myofibroblast Contraction: Role of Rho, Myosin Light Chain Kinase, and Myosin Light Chain PhosphataseExperimental Cell Research, 2000