NG2 Glia Generate New Oligodendrocytes But Few Astrocytes in a Murine Experimental Autoimmune Encephalomyelitis Model of Demyelinating Disease
Open Access
- 1 December 2010
- journal article
- research article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 30 (48), 16383-16390
- https://doi.org/10.1523/jneurosci.3411-10.2010
Abstract
The adult mammalian brain and spinal cord contain glial precursors that express platelet-derived growth factor receptor α subunit (PDGFRA) and the NG2 proteoglycan. These “NG2 cells” descend from oligodendrocyte precursors in the perinatal CNS and continue to generate myelinating oligodendrocytes in the gray and white matter of the postnatal brain. It has been proposed that NG2 cells can also generate reactive astrocytes at sites of CNS injury or demyelination. To test this we examined the fates of PDGFRA/NG2 cells in the mouse spinal cord during experimental autoimmune encephalomyelitis (EAE)—a demyelinating condition that models some aspects of multiple sclerosis in humans. We administered tamoxifen toPdgfra-CreERT2:Rosa26R-YFPmice to induce yellow fluorescent protein (YFP) expression in PDGFRA/NG2 cells and their differentiated progeny. We subsequently induced EAE and observed a large (>4-fold) increase in the local density of YFP+cells, >90% of which were oligodendrocyte lineage cells. Many of these became CC1-positive, NG2-negative differentiated oligodendrocytes that expressed myelin markers CNP and Tmem10/Opalin. PDGFRA/NG2 cells generated very few GFAP+-reactive astrocytes (1–2% of all YFP+cells) or NeuN+neurons (<0.02%). Thus, PDGFRA/NG2 cells act predominantly as a reservoir of new oligodendrocytes in the demyelinated spinal cord.Keywords
This publication has 49 references indexed in Scilit:
- Pyramidal Neurons Are Generated from Oligodendroglial Progenitor Cells in Adult Piriform CortexJournal of Neuroscience, 2010
- NG2 cells differentiate into astrocytes in cerebellar slicesMolecular and Cellular Neuroscience, 2009
- Early Postnatal Proteolipid Promoter-Expressing Progenitors Produce Multilineage CellsIn VivoJournal of Neuroscience, 2009
- Postinjury Niches Induce Temporal Shifts in Progenitor Fates to Direct Lesion Repair after Spinal Cord InjuryJournal of Neuroscience, 2009
- Age‐related myelin dynamics revealed by increased oligodendrogenesis and short internodesAging Cell, 2009
- Neurogenesis in the chronic lesions of multiple sclerosisBrain, 2008
- Identification of Tmem10/Opalin as an oligodendrocyte enriched gene using expression profiling combined with genetic cell ablationGlia, 2008
- Neuroprotection by tamoxifen in focal cerebral ischemia is not mediated by an agonist action at estrogen receptors but is associated with antioxidant activityExperimental Neurology, 2007
- Fate of endogenous stem/progenitor cells following spinal cord injuryJournal of Comparative Neurology, 2006
- Development of NG2 neural progenitor cells requires Olig gene functionProceedings of the National Academy of Sciences of the United States of America, 2006