Spatial and temporal patterns of cell death in limb bud mesoderm after apical ectodermal ridge removal
- 1 September 1982
- journal article
- research article
- Published by Elsevier BV in Developmental Biology
- Vol. 93 (1), 83-91
- https://doi.org/10.1016/0012-1606(82)90241-x
Abstract
A spatiotemporal pattern of cell death occurred in the chick wing and leg bud mesoderm after removal of apical ectodermal ridge at stages 18–20. Cells died in a region extending from the limb bud distal surface to 150–200 μm into the mesoderm. Limb buds from which ridge was removed at later stages in development did not exhibit a spatiotemporal pattern of cell death. In control experiments in which dorsal ectoderm was removed, a pattern of cell death did not occur. Removal of the ridge and part of the 150- to 200-μm zone of prospective cell death resulted in cell death in an area approximately equal to the amount of the zone remaining. After removal of all of the prospective zone of cell death plus the apical ridge, cell death was observed in the remaining limb bud mesoderm. In these limb buds, cell death occurred in a region in which it had not been seen in limb bud with apical ridge alone removed. We conclude that at stages 18–20 the mesodermal cells 150–200 μm beneath the ridge require the apical ridge to survive. More proximal mesodermal cells do not die after ridge removal alone, but apparently require the presence of the more distal mesoderm to survive. Whether this is a requirement for something intrinsic to the distal mesoderm or something it possesses by way of the ridge is unknown. After stage 23, the limb mesoderm cells do not die when the apical ridge is removed. Nevertheless, at the later stages, ridge continues to be required for limb bud proximal-distal elongation and the differentiation of distal limb elements.This publication has 14 references indexed in Scilit:
- Effects of excising the apical ectodermal ridge on the development of the marginal vasculature of the wing bud in the chick embryoJournal of Experimental Zoology, 1982
- Pattern Specification and Pattern Regulation in the Embryonic Chick Limb BudAmerican Zoologist, 1982
- Development of the embryonic chick limb bud in avascular cultureDevelopmental Biology, 1968
- Regeneration of the apical ectodermal ridge of the chick limb budDevelopmental Biology, 1964
- Development of fragmented and of dissociated limb bud mesodermDevelopmental Biology, 1964
- Cellular death in morphogenesis of the avian wingDevelopmental Biology, 1962
- Effects of the apical ectodermal ridge on growth of the versene-stripped chick limb budDevelopmental Biology, 1961
- Determination of structural patterns in the spinal cord of the chick embryo studied by transplantations between brachial and adjacent levelsJournal of Experimental Zoology, 1951
- The proximo‐distal sequence of origin of the parts of the chick wing and the role of the ectodermJournal of Experimental Zoology, 1948
- Experimental studies on the development of the wing in the embryo of Gallus domesticusJournal of Anatomy, 1934