Characterization of a Novel Potyvirus Isolated from Maize in Israel
Open Access
- 1 May 2000
- journal article
- Published by Scientific Societies in Phytopathology®
- Vol. 90 (5), 505-513
- https://doi.org/10.1094/phyto.2000.90.5.505
Abstract
A potyvirus (proposed name of Zea mosaic virus [ZeMV]) isolated from maize in Israel was analyzed by serology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of capsid proteins, symptomatology, and sequencing. Parts of the nuclear inclusion b, coat protein, and 3′ regions were sequenced; the amino acid sequence of ZeMV capsid was determined by time-of-flight mass spectrometry (TOFMS). The results of these analyses were compared with those of similar analyses of the following potyviruses: Maize dwarf mosaic virus (MDMV), Sugarcane mosaic virus strain MDB (SCMV-MDB), Johnsongrass mosaic virus(JGMV), Sorghum mosaic virus (SrMV), and an isolate of MDMV from Israel. Indirect enzyme-linked immunosorbent assay using ZeMV antiserum detected only ZeMV, and reciprocal tests using MDMV, JGMV, or SrMV antisera failed to detect ZeMV. ZeMV cross-reacted weakly when SCMV-MDB antiserum was used. The mass of ZeMV capsid was determined to be 36,810 Da by SDS-PAGE and 34,216 Da by TOFMS. The ZeMV systemically infected johnsongrass (Sorghum halepense), but did not infect oat (Avena sativa), pearl millet (Pennisetum glaucum), barley (Hordeum vulgare), or rye (Secale cereale). Necrosis was caused in 19 sorghum lines by SrMV, in 15 by ZeMV, in 14 by MDMV, and in 5 by JGMV and SCMV-MDB. The nucleic acid and amino acid sequences of ZeMV clearly showed that it is not a strain of JGMV, MDMV, SCMV, or SrMV.Keywords
This publication has 40 references indexed in Scilit:
- Comparison of the complete nucleotide sequences of two isolates of lettuce mosaic virus differing in their biological propertiesVirus Research, 1997
- Natural Infection of Pearl Millet and Sorghum by Wheat Streak Mosaic Virus in KansasPlant Disease, 1996
- Occurrence and Symptom Expression of American Wheat Striate Mosaic Virus in Wheat in KansasPlant Disease, 1995
- Partial Resistance of Sugary Enhancer Sweet Corn Genotypes to Two Isolates of the Sugarcane Mosaic Subgroup of PotyvirusesPlant Disease, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Sequence analysis of the 3′-terminal half of RNA 1 of wheat spindle streak mosaic virusArchiv für die gesamte Virusforschung, 1994
- Taxonomy of Potyviruses Infecting Maize, Sorghum, and Sugarcane in Australia and the United States as Determined by Reactivities of Polyclonal Antibodies Directed towards Virus-Specific N-Termini of Coat ProteinsPhytopathology®, 1989
- Nucleotide sequence of the 3' terminal region of plum pox potyvirus RNAVirus Research, 1988
- Size Variation Among Proteins Induced by Sugarcane Mosaic Viruses in Plant TissuePhytopathology®, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970