A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier
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- 1 June 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 292 (5522), 1722-1725
- https://doi.org/10.1126/science.1059852
Abstract
Listeria monocytogenes is responsible for severe food-borne infections, but the mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria monocytogenes expresses a surface protein, internalin, that interacts with a host receptor, E-cadherin, to promote entry into human epithelial cells. Murine E-cadherin, in contrast to guinea pig E-cadherin, does not interact with internalin, excluding the mouse as a model for addressing internalin function in vivo. In guinea pigs and transgenic mice expressing human E-cadherin, internalin was found to mediate invasion of enterocytes and crossing of the intestinal barrier. These results illustrate how relevant animal models for human infections can be generated.Keywords
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