Resistance to Erysiphe necator in the grapevine ‘Kishmish vatkana’ is controlled by a single locus through restriction of hyphal growth
- 7 December 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 116 (3), 427-438
- https://doi.org/10.1007/s00122-007-0680-4
Abstract
Vitis vinifera ‘Kishmish vatkana’, a cultivated grapevine from Central Asia, does not produce visible symptoms in response to natural or artificial inoculation with the fungus Erysiphe necator Schwein., the casual agent of powdery mildew. ‘Kishmish vatkana’ allowed pathogen entry into epidermal cells at a rate comparable to that in the susceptible control Vitis vinifera ‘Nimrang’, but was able to limit subsequent hyphal proliferation. Density of conidiophores was significantly lower in ‘Kishmish vatkana’ (33.6 ± 8.7 conidiophores mm−2) than in ‘Nimrang’ (310.5 ± 24.0 conidiophores mm−2) by 120 h after inoculation. A progeny of 310 plants from a ‘Nimrang’ × ‘Kishmish vatkana’ cross were scored for the presence or absence of visible conidiophores throughout two successive seasons. Phenotypic segregation revealed the presence of a single dominant allele termed Resistance to Erysiphe necator 1 (REN1), which was heterozygous in ‘Kishmish vatkana’. A bulked segregant analysis was carried out using 195 microsatellite markers uniformly distributed across the entire genome. For each marker, association with the resistance trait was inferred by measuring in the bulks the ratio of peak intensities of the two alleles inherited from ‘Kishmish vatkana’. The phenotypic locus was assigned to linkage group 13, a genomic region in which no disease resistance had been reported previously. The REN1 position was restricted to a 7.4 cM interval by analyzing the 310 offspring for the segregation of markers that surrounded the target region. The closest markers, VMC9H4-2, VMCNG4E10-1 and UDV-020, were located 0.9 cM away from the REN1 locus.Keywords
This publication has 27 references indexed in Scilit:
- Powdery Mildew Induces Defense-Oriented Reprogramming of the Transcriptome in a Susceptible But Not in a Resistant GrapevinePlant Physiology, 2007
- An integrated SSR map of grapevine based on five mapping populationsTheoretical and Applied Genetics, 2006
- Arabidopsis SENESCENCE-ASSOCIATED GENE101 Stabilizes and Signals within an ENHANCED DISEASE SUSCEPTIBILITY1 Complex in Plant Innate ImmunityPlant Cell, 2005
- CARHTA GENE: multipopulation integrated genetic and radiation hybrid mappingBioinformatics, 2004
- Sensitivity of California Isolates of Uncinula necator to Trifloxystrobin and Spiroxamine, and Update on Triadimefon SensitivityPlant Disease, 2004
- SNARE-protein-mediated disease resistance at the plant cell wallNature, 2003
- PATTERNS OF PESTICIDE USE IN CALIFORNIA AND THE IMPLICATIONS FOR STRATEGIES FOR REDUCTION OF PESTICIDESAnnual Review of Phytopathology, 2003
- The effects of allelic variation at the Mla resistance locus in barley on the early development of Erysiphe graminis f.sp. hordei and host responsesThe Plant Journal, 1995
- Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proceedings of the National Academy of Sciences, 1991
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987