Lack of colonization of 1 day old chicks by viable, non‐culturable Campylobacter jejuni
- 1 June 1992
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 72 (6), 512-516
- https://doi.org/10.1111/j.1365-2672.1992.tb01868.x
Abstract
Seven strains of Campylobacter jejuni, isolated from various sources [human (n = 2), chicken (n = 3), water (n = 2)], were studied under starvation conditions in filter-sterilized and pasteurized surface water by acridine orange direct count (AODC), viable count (DVC) and culture methods. Plate counts showed a rapid decline (2 log-units/day) for all strains under these conditions. Only one of the seven strains (14%) showed a (prolonged) viable, non-culturable 'state'. The ability of these viable, non-culturable cells to colonize the intestine was tested on day-old chicks. The infectious oral dose of freshly cultured cells of this model was 26-260 cfu; 1.8 x 10(5) viable, non-culturable C. jejuni were introduced to day-old chicks orally. Campylobacter jejuni was not isolated from the caeca of the chicks after incubation for 7 d. Also, passage through the allantoic fluid of embryonated eggs did not recover viable, non-culturable C. jejuni. These findings cast serious doubts on the significance of the viable, non-culturable 'state' in environmental transmission of C. jejuni.Keywords
This publication has 21 references indexed in Scilit:
- Effect of Heat Shock on Recovery of Escherichia coli from Drinking WaterWater Science & Technology, 1991
- Legionella and Public Water SuppliesWater Science & Technology, 1988
- Viable Legionella pneumophila Not Detectable by Culture on Agar MediaNature Biotechnology, 1987
- Viability and virulençe ofEscherichia colisuspended by membrane chamber in semitropical ocean waterFEMS Microbiology Letters, 1986
- Viable but Non-Culturable Vibrio cholerae and Related Pathogens in the Environment: Implications for Release of Genetically Engineered MicroorganismsNature Biotechnology, 1985
- Experimental Gastroenteritis in Newly-Hatched Chicks Infected with Campylobacter JejuniJournal of Medical Microbiology, 1984
- Viable but nonrecoverable stage of Salmonella enteritidis in aquatic systemsCanadian Journal of Microbiology, 1984
- Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environmentMicrobial Ecology, 1982
- A tentative direct microscopic method for counting living marine bacteriaCanadian Journal of Microbiology, 1979
- Use of nuclepore filters for counting bacteria by fluorescence microscopyApplied and Environmental Microbiology, 1977