P‐glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated
- 6 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 257 (2), 419-421
- https://doi.org/10.1016/0014-5793(89)81586-8
Abstract
There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (M r 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (M r 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (M r 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.Keywords
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