Energy-dissipative supercomplex of photosystem II associated with LHCSR3 in Chlamydomonas reinhardtii
- 28 May 2013
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 110 (24), 10016-10021
- https://doi.org/10.1073/pnas.1222606110
Abstract
Plants and green algae have a low pH-inducible mechanism in photosystem II (PSII) that dissipates excess light energy, measured as the nonphotochemical quenching of chlorophyll fluorescence (qE). Recently, nonphotochemical quenching 4 (npq4), a mutant strain of the green alga Chlamydomonas reinhardtii that is qE-deficient and lacks the light-harvesting complex stress-related protein 3 (LHCSR3), was reported [Peers G, et al. (2009) Nature 462(7272):518–521]. Here, applying a newly established procedure, we isolated the PSII supercomplex and its associated light-harvesting proteins from both WT C. reinhardtii and the npq4 mutant grown in either low light (LL) or high light (HL). LHCSR3 was present in the PSII supercomplex from the HL-grown WT, but not in the supercomplex from the LL-grown WT or mutant. The purified PSII supercomplex containing LHCSR3 exhibited a normal fluorescence lifetime at a neutral pH (7.5) by single-photon counting analysis, but a significantly shorter lifetime at pH 5.5, which mimics the acidified lumen of the thylakoid membranes in HL-exposed chloroplasts. The switch from light-harvesting mode to energy-dissipating mode observed in the LHCSR3-containing PSII supercomplex was sensitive to dicyclohexylcarbodiimide, a protein-modifying agent specific to protonatable amino acid residues. We conclude that the PSII-LHCII-LHCSR3 supercomplex formed in the HL-grown C. reinhardtii cells is capable of energy dissipation on protonation of LHCSR3.Keywords
This publication has 46 references indexed in Scilit:
- Revisiting the Supramolecular Organization of Photosystem II in Chlamydomonas reinhardtiiPublished by Elsevier BV ,2012
- Fluorescence lifetime snapshots reveal two rapidly reversible mechanisms of photoprotection in live cells of Chlamydomonas reinhardtiiProceedings of the National Academy of Sciences of the United States of America, 2012
- Photoprotective Energy Dissipation Involves the Reorganization of Photosystem II Light-Harvesting Complexes in the Grana Membranes of Spinach ChloroplastsPlant Cell, 2011
- Analysis of LhcSR3, a Protein Essential for Feedback De-Excitation in the Green Alga Chlamydomonas reinhardtiiPLoS Biology, 2011
- An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to lightProceedings of the National Academy of Sciences of the United States of America, 2010
- Photoprotection in the diatom Thalassiosira pseudonana: Role of LI818-like proteins in response to high light stressBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2010
- Physcomitrella patens mutants affected on heat dissipation clarify the evolution of photoprotection mechanisms upon land colonizationProceedings of the National Academy of Sciences of the United States of America, 2010
- Molecular Remodeling of Photosystem II during State Transitions inChlamydomonas reinhardtiiPlant Cell, 2008
- In Silico and Biochemical Analysis of Physcomitrella patens Photosynthetic Antenna: Identification of Subunits which Evolved upon Land AdaptationPLOS ONE, 2008
- Interactions between the Photosystem II Subunit PsbS and Xanthophylls Studied in Vivo and in VitroPublished by Elsevier BV ,2008