Use of highly sensitive mitochondrial probes to detect microchimerism in xenotransplantation models
- 1 January 2006
- journal article
- research article
- Published by Wiley in Xenotransplantation
- Vol. 13 (1), 80-85
- https://doi.org/10.1111/j.1399-3089.2005.00256.x
Abstract
Chimerism, defined as the co-existence of cells of different origin within the same organism, has received much attention in hematopoietic cell and organ transplantation because of the strict relationship between its establishment and the induction of specific tolerance. Traditional methods applied for chimerism detection, such as immunohistochemistry, cytogenetics, fluorescent-activated cell sorter analysis, and serological and biochemical testing, are limited by their sensitivity. We have established a highly sensitive molecular approach based on the amplification of the mitochondrial cytochrome B gene and tested its specificity and sensitivity level in six different mammalian species, including human, pig, mouse, rat, sheep and rabbit. Increased sensitivity of detection of specific amplification products was obtained by the non-radioactive Southern blot technique. This novel approach allows the detection of one cell against the background of 1 to 4 x 10(6) xenogenec cells and will be helpful for high-sensitivity analysis of donor cell engraftment after xenotransplantation procedures in these animal modelsKeywords
This publication has 29 references indexed in Scilit:
- Outcome of hematopoietic stem cell transplantation in patients with atypical chronic myeloid leukemiaBone Marrow Transplantation, 2004
- Multilineage hematopoietic engraftment after allogeneic peripheral blood stem cell transplantation without conditioning in SCID patientsBone Marrow Transplantation, 2004
- Human Pancreatic Islet-Derived Progenitor Cell Engraftment in Immunocompetent MiceThe American Journal of Pathology, 2004
- Complete allogeneic hematopoietic chimerism achieved by a combined strategy of in utero hematopoietic stem cell transplantation and postnatal donor lymphocyte infusionBlood, 2002
- Isolation and characterization of canine hematopoietic progenitor cellsExperimental Hematology, 2001
- Cellular Chimerism of the Lung After TransplantationAmerican Journal of Clinical Pathology, 2001
- Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheepNature Medicine, 2000
- A POLYMERASE CHAIN REACTION-BASED PROTOCOL FOR THE DETECTION OF TRANSMISSION OF PIG ENDOGENOUS RETROVIRUSES IN PIG TO HUMAN XENOTRANSPLANTATION1Transplantation, 2000
- Search for Cross-Species Transmission of Porcine Endogenous Retrovirus in Patients Treated with Living Pig TissueScience, 1999
- HIGHLY SENSITIVE AND SPECIFIC POLYMERASE CHAIN REACTION ASSAYS FOR DETECTION OF BABOON AND PIG CELLS FOLLOWING XENOTRANSPLANTATION IN HUMANSTransplantation, 1996