A Distinct G 1 Step Required to Specify the Chinese Hamster DHFR Replication Origin
- 1 March 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5253), 1270-1272
- https://doi.org/10.1126/science.271.5253.1270
Abstract
Nuclei isolated from Chinese hamster ovary (CHO) cells at various times during the G1 phase of the cell cycle were stimulated to enter S phase by incubation in Xenopus egg cytosol. Replication of DNA initiated within the dihydrofolate reductase (DHFR) origin locus in nuclei isolated late in G1, but at random sites in nuclei isolated early in G1. A discrete transition point occurred 3 to 4 hours after metaphase. Neither replication licensing nor nuclear assembly was sufficient for origin recognition. Thus, a distinct cell cycle-regulated event in the nucleus restricts the initiation of replication to specific sites downstream of the DHFR gene.Keywords
This publication has 13 references indexed in Scilit:
- The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extractsCurrent Biology, 1995
- MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cellsNature, 1995
- Purification of an MCM-containing complex as a component of the DNA replication licensing systemNature, 1995
- Identification of the yeast MCM3-related protein as a component of xenopus DNA replication licensing factorCell, 1995
- Defining origins of replication in mammalian cellsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1994
- Two steps in the assembly of complexes at yeast replication origins in vivoCell, 1994
- REGULATION OF EUKARYOTIC DNA REPLICATIONAnnual Review of Biochemistry, 1994
- DNA Replication Origins in Animal Cells: a Question of Context?Science, 1994
- The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extractCell, 1992
- G 1 Events and Regulation of Cell ProliferationScience, 1989