Matrix Sublimation/Recrystallization for Imaging Proteins by Mass Spectrometry at High Spatial Resolution
- 3 June 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 83 (14), 5728-5734
- https://doi.org/10.1021/ac200998a
Abstract
We have employed matrix deposition by sublimation for protein image analysis on tissue sections using a hydration/recrystallization process that produces high-quality MALDI mass spectra and high-spatial-resolution ion images. We systematically investigated different washing protocols, the effect of tissue section thickness, the amount of sublimated matrix per unit area, and different recrystallization conditions. The results show that an organic solvent rinse followed by ethanol/water rinses substantially increased sensitivity for the detection of proteins. Both the thickness of the tissue section and the amount of sinapinic acid sublimated per unit area have optimal ranges for maximal protein signal intensity. Ion images of mouse and rat brain sections at 50, 20, and 10 μm spatial resolution are presented and are correlated with hematoxylin and eosin (H&E)-stained optical images. For targeted analysis, histology-directed imaging can be performed using this protocol where MS analysis and H&E staining are performed on the same section.Keywords
This publication has 16 references indexed in Scilit:
- Revisiting Rat Spermatogenesis with MALDI Imaging at 20-μm ResolutionMolecular & Cellular Proteomics, 2011
- Matrix vapor deposition/recrystallization and dedicated spray preparation for high‐resolution scanning microprobe matrix‐assisted laser desorption/ionization imaging mass spectrometry (SMALDI‐MS) of tissue and single cellsRapid Communications in Mass Spectrometry, 2010
- MALDI Imaging Mass SpectrometryMolecular & Cellular Proteomics, 2009
- A mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitorsRapid Communications in Mass Spectrometry, 2009
- Enhancement of protein sensitivity for MALDI imaging mass spectrometry after chemical treatment of tissue sectionsJournal of the American Society for Mass Spectrometry, 2008
- Sublimation as a method of matrix application for mass spectrometric imagingJournal of the American Society for Mass Spectrometry, 2007
- Automated MALDI Matrix Deposition Method with Inkjet Printing for Imaging Mass SpectrometryAnalytical Chemistry, 2007
- New Developments in Profiling and Imaging of Proteins from Tissue Sections by MALDI Mass SpectrometryJournal of Proteome Research, 2006
- A Novel Histology-directed Strategy for MALDI-MS Tissue Profiling That Improves Throughput and Cellular Specificity in Human Breast CancerMolecular & Cellular Proteomics, 2006
- Peer Reviewed: Profiling and Imaging Proteins in Tissue Sections by MSAnalytical Chemistry, 2004