A protein translocation defect linked to ubiquitin conjugation at the endoplasmic reticulum
- 9 September 1993
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 365 (6442), 176-179
- https://doi.org/10.1038/365176a0
Abstract
UBIQUITIN-conjugating enzymes function in selective proteolysis pathways and catalyse the covalent attachment of ubiquitin to proteolytic substrates1–4. Here we report the identification of an integral membrane ubiquitin-conjugating enzyme. This enzyme, UBC6, localizes to the endoplasmic reticulum (ER), with the cata-lytic domain facing the cytosol. ubc6 loss-of-function mutants sup-press the protein translocation defect caused by a mutation in SEC61, which encodes a key component of a multisubunit protein translocation apparatus of the ER5–11. The expression of the sec61 mutant phenotype requires both the activity of UBC6 and its localization at the ER membrane. This suggests that UBC6 may mediate selective degradation of ER membrane proteins and that the protein translocation defect of sec61 may be caused by proteolysis of components of a structurally distorted mutant translocation apparatus.This publication has 30 references indexed in Scilit:
- Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MATα2 repressorCell, 1993
- The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymesNature, 1992
- Ubiquitin-dependent protein degradation: a cellular perspectiveTrends in Cell Biology, 1992
- Sec61p and BiP directly facilitate polypeptide translocation into the ERCell, 1992
- Yeast Sec proteins interact with polypeptides traversing the endoplasmic reticulum membraneCell, 1992
- The ubiquitin pathway for protein degradationTrends in Biochemical Sciences, 1991
- Degradation of proteins within the endoplasmic reticulumCurrent Opinion in Cell Biology, 1991
- The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.Proceedings of the National Academy of Sciences of the United States of America, 1991
- Protein degradation in the endoplasmic reticulumCell, 1990
- The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzymeNature, 1987