Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry
Open Access
- 15 February 1998
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 12 (4), 456-461
- https://doi.org/10.1101/gad.12.4.456
Abstract
To understand the mechanisms by which CDKs regulate cell cycle progression, it is necessary to identify and characterize the physiological substrates of these kinases. We have developed a screening method to identify novel CDK substrates. One of the cDNAs identified in the screen is identical to the recently isolatedNPAT gene. Here we show that NPAT associates with cyclin E–CDK2 in vivo and can be phosphorylated by this CDK. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry, and this effect is enhanced by coexpression of cyclin E–CDK2. These results suggest that NPAT is a substrate of cyclin E–CDK2 and plays a role in S-phase entry.Keywords
This publication has 30 references indexed in Scilit:
- Cancer Cell CyclesScience, 1996
- Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinaseCell, 1994
- Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signalsCell, 1994
- Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system.Molecular and Cellular Biology, 1994
- Distinct Roles for Cyclin-Dependent Kinases in Cell Cycle ControlScience, 1993
- Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins.Genes & Development, 1993
- The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E.Genes & Development, 1993
- The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinasesCell, 1993
- The cdk2 kinase is required for the G1-to-S transition in mammalian cells.1993
- Cyclin-Dependent Regulation of G 1 in Mammalian FibroblastsScience, 1993