Rapid detection, enrichment and propagation of specific T cell subsets based on cytokine secretion
Open Access
- 21 October 2010
- journal article
- review article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 163 (1), 1-10
- https://doi.org/10.1111/j.1365-2249.2010.04261.x
Abstract
T cell lines with defined cytokine profiles are an invaluable tool for assessing the control of immune responses both in vitro and in vivo. Production of such cell lines can be complex and time-consuming. Here we present a powerful technique to assay the cytokines produced by T cells activated polyclonally or with specific antigens. This paper presents a detailed methodology for the identification and isolation of cytokine-producing T cells activated with the artificial superantigen, CytoStim, or viral and fungal antigens. These cells can be analysed for different cytokines simultaneously, or cultured further to rapidly establish T cell lines making known cytokine types. We highlight the enumeration, isolation and phenotype of interleukin-17-producing T cells, and the rapid generation of virus-specific Th1 T cell lines.Keywords
This publication has 12 references indexed in Scilit:
- Isolation and characterization of human interleukin-10–secreting T cells from peripheral bloodHuman Immunology, 2010
- Cytomegalovirus‐Specific T Cell Immunotherapy Promotes Restoration of Durable Functional Antiviral Immunity following Allogeneic Stem Cell TransplantationClinical Infectious Diseases, 2009
- Translational Mini-Review Series on Th17 Cells: CD4+ T helper cells: functional plasticity and differential sensitivity to regulatory T cell-mediated regulationClinical and Experimental Immunology, 2009
- Cutting Edge: Th1 Cells Facilitate the Entry of Th17 Cells to the Central Nervous System during Experimental Autoimmune EncephalomyelitisThe Journal of Immunology, 2008
- Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primatesJCI Insight, 2008
- Surface phenotype and antigenic specificity of human interleukin 17–producing T helper memory cellsNature Immunology, 2007
- Rapid generation of combined CMV-specific CD4+ and CD8+ T-cell lines for adoptive transfer into recipients of allogeneic stem cell transplantsBlood, 2004
- Detection and enrichment of antigen-specific CD4+ and CD8+ T cells based on cytokine secretionMethods, 2003
- Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix.Proceedings of the National Academy of Sciences of the United States of America, 1995
- Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.The Journal of Immunology, 1986