Corneal epithelial nuclear ferritin: Developmental regulation of ferritin and its nuclear transporter ferritoid
Open Access
- 26 August 2008
- journal article
- patterns and-phenotypes
- Published by Wiley in Developmental Dynamics
- Vol. 237 (9), 2529-2541
- https://doi.org/10.1002/dvdy.21691
Abstract
The corneal epithelium is exposed to reactive oxygen species that are potentially deleterious to nuclear DNA. However, our previous studies show that corneal epithelial cells have a novel, developmentally regulated mechanism for protection from such damage that involves having the iron‐sequestering molecule, ferritin, in the nucleus. Nuclear localization of ferritin is achieved through the action of a tissue‐specific nuclear transporter, ferritoid, which is itself a ferritin family member. Here, we show that during development ferritoid appears before ferritin. At this time, ferritoid is cytoplasmic, suggesting that its nuclear transport function requires an interaction with ferritin. To examine the developmental regulation of these two interacting components, cultured corneas were treated with the iron chelator deferoxamine. The results show that, while iron‐mediated translational regulation is involved in the synthesis of both molecules, ferritoid is also transcriptionally regulated, demonstrating that these family members—whose functions depend upon one another—are regulated differently. Developmental Dynamics 237:2529–2541, 2008.Keywords
This publication has 26 references indexed in Scilit:
- Nuclear ferritin‐mediated protection of corneal epithelial cells from oxidative damage to DNADevelopmental Dynamics, 2008
- Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahsAvian Pathology, 2005
- Ferritoid, a Tissue-specific Nuclear Transport Protein for Ferritin in Corneal Epithelial CellsOnline Journal of Public Health Informatics, 2003
- Iron Homeostasis, Oxidative Stress, and DNA DamageFree Radical Biology & Medicine, 1997
- Ferritin Is a Developmentally Regulated Nuclear Protein of Avian Corneal Epithelial CellsOnline Journal of Public Health Informatics, 1997
- Oxidative Damage to DNA Constituents by Iron-mediated Fenton ReactionsOnline Journal of Public Health Informatics, 1996
- The ferritins: molecular properties, iron storage function and cellular regulationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1996
- Oxidative mechanisms in the toxicity of metal ionsFree Radical Biology & Medicine, 1995
- American journal of anatomy: 1901-1991Journal of Anatomy, 1991