A genetic system for analysis of staphylococcal nuclease
- 30 June 1983
- journal article
- Published by Elsevier BV in Gene
- Vol. 22 (2-3), 181-189
- https://doi.org/10.1016/0378-1119(83)90102-6
Abstract
The gene encoding staphylococcal nuclease (strain Foggi) has been isolated, and its complete nucleotide sequence determined. When inserted into a derivative of plasmid pBR322, the nuc gene is expressed in Escherichia coli at a low level, and nuclease activity in individual colonies is readily assayed by a replica-plating chromogenic test. A set of plasmids with BamHI-linker “bracketed” deletions spanning the nuc gene can be used to map mutations genetically to defined segments of the gene. In combination with present methods for efficient in vitro mutagenesis, this plasmid-based genetic system can be applied to the detailed genetic analysis of this small, biochemically and biophysically well-characterized enzyme.Keywords
This publication has 13 references indexed in Scilit:
- Mechanism of Incorporation of Cell Envelope Proteins in Escherichia ColiAnnual Review of Microbiology, 1982
- DIRECTED MUTAGENESISAnnual Review of Genetics, 1981
- DNA sequence analysis of the transposon Tn3: Three genes and three sites involved in transposition of Tn3Cell, 1979
- Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymologyMolecular and Cellular Biochemistry, 1979
- Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymologyMolecular and Cellular Biochemistry, 1979
- Staphylococcal nuclease reviewed: A Prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding.Molecular and Cellular Biochemistry, 1979
- Prolonged incubation in calcium chloride improves the competence of Escherichia coli cellsGene, 1979
- Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymology. I isolation; physical and enzymatic propertiesMolecular and Cellular Biochemistry, 1978
- Conversion of closed circular DNA molecules to single-nicked molecules by digestion with DNAase I in the presence of ethidium bromideBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Mapping temperature-sensitive mutants of simian virus 40: Rescue of mutants by fragments of viral DNAVirology, 1974