Eutectic Phases in Ice Facilitate Nonenzymatic Nucleic Acid Synthesis
- 1 September 2001
- journal article
- research article
- Published by Mary Ann Liebert Inc in Astrobiology
- Vol. 1 (3), 271-281
- https://doi.org/10.1089/15311070152757465
Abstract
Polymeric compounds similar to oligonucleotides are relevant to the origin of life and particularly to the concept of an RNA world. Although short oligomers of RNA can be synthesized nonenzymatically under laboratory conditions by second-order reactions in concentrated solutions, there is no consensus on how these polymers could have been synthesized de novo on the early Earth from dilute solutions of monomers. To address this question in the context of an RNA world, we have explored ice eutectic phases as a reaction medium. When an aqueous solution freezes, the solutes become concentrated in the spaces between the ice crystals. The increased concentration offsets the effect of the lower temperature and accelerates the reaction. Here we show that in the presence of metal ions in dilute solutions, frozen samples of phosphoimidazolide-activated uridine react within days at -18°C to form oligouridylates up to 11 bases long. Product yields typically exceed 90%, and ~30% of the oligomers include one or more 3′-5′ linkages. These conditions facilitate not only the notoriously difficult oligouridylate synthesis, but also the oligomerization of activated cytidylate, adenylate, and guanylate. To our knowledge, this represents the first report to indicate that ice matrices on the early Earth may have accelerated certain prebiotic polymerization reactions.Keywords
This publication has 52 references indexed in Scilit:
- Sequence- and Regio-Selectivity in the Montmorillonite-Catalyzed Synthesis of RNAOrigins of life and evolution of the biosphere, 2000
- Bridging the Prebiotic and RNA Worlds: Prebiotic RNA Synthesis on ClayJournal of Biomolecular Structure and Dynamics, 2000
- Prebiotic synthesis on minerals: bridging the prebiotic and RNA worlds.The Biological Bulletin, 1999
- Synthesis of long prebiotic oligomers on mineral surfacesNature, 1996
- Oligomerization of uridine phosphorimidazolides on montmorillonite: A model for the prebiotic synthesis of rna on mineralsOrigins of life and evolution of the biosphere, 1996
- Impact melting of frozen oceans on the early Earth: Implications for the origin of lifeProceedings of the National Academy of Sciences of the United States of America, 1994
- The limits of template-directed synthesis with nucleoside-5′-phosphoro(2-methyl)imidazolidesOrigins of life and evolution of the biosphere, 1993
- Chemical ligation of oligonucleotides in the presence and absence of a templateJournal of the American Chemical Society, 1993
- The adsorption of nucleotides and polynucleotides on montmorillonite clayOrigins of life and evolution of the biosphere, 1989
- Origin of life: The RNA worldNature, 1986