Molybdate and tungstate act like vanadate on glucose metabolism in isolated hepatocytes
- 15 March 1992
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 282 (3), 659-663
- https://doi.org/10.1042/bj2820659
Abstract
In rat hepatocytes, molybdate and tungstate inactivate glycogen synthase by a mechanism independent of Ca2+ and activate glycogen phosphorylase by a Ca(2+)-dependent mechanism. On the other hand, both molybdate and tungstate increase fructose 2,6-bisphosphate levels and counteract the decrease in this metabolite induced by glucagon. These effectors do not directly modify 6-phosphofructo-2-kinase activity, even though they partially counteract the inactivation of this enzyme induced by glucagon. These effects are related to an increase on the glycolytic flux, as indicated by the increase in L-lactate and CO2 production and the decrease in glucose 6-phosphate levels in the presence of glucose. All these effects are similar to those previously reported for vanadate, although molybdate and tungstate are less effective than vanadate. These results could indicate that molybdate, tungstate and vanadate act on glucose metabolism in isolated hepatocytes by a similar mechanism of action.Keywords
This publication has 24 references indexed in Scilit:
- Activation by Vanadate of Glycolysis in Hepatocytes From Diabetic RatsDiabetes, 1991
- Oral administration of vanadate to streptozotocin-diabetic rats restores the glucose-induced activation of liver glycogen synthaseBiochemical Journal, 1990
- Long Term Improvement of Glucose Homeostasis by Vanadate in Obese Hyperinsulinemic fa/fa Rats*Endocrinology, 1989
- Vanadate raises fructose 2,6-bisphosphate concentrations and activates glycolysis in rat hepatocytes.1988
- Inhibition of phosphatase and sulfatase by transition-state analogsBiochemistry, 1988
- Oral administration of vanadate normalizes blood glucose levels in streptozotocin-treated rats. Characterization and mode of action.Journal of Biological Chemistry, 1987
- Glycogenolytic, noninsulin-like effects of vanadate on rat hepatocyte glycogen synthase and phosphorylase.Journal of Biological Chemistry, 1986
- Insulin-like stimulation of glucose oxidation in rat adipocytes by vanadyl (IV) ionsNature, 1980
- Evidence for the Involvement of Sulfhydryl Oxidation in the Regulation of Fat Cell Hexose Transport by InsulinProceedings of the National Academy of Sciences of the United States of America, 1974