Recent Advances and Achievements in Nanomaterial-Based, and Structure Switchable Aptasensing Platforms for Ochratoxin A Detection
Open Access
- 5 November 2013
- Vol. 13 (11), 15187-15208
- https://doi.org/10.3390/s131115187
Abstract
Aptamer-based bioreceptors that can easily adopt their surroundings have captured the attention of scientists from a wide spectrum of domains in designing highly sensitive, selective and structure switchable sensing assays. Through elaborate design and chemical functionalization, numerous aptamer-based assays have been developed that can switch their conformation upon incubation with target analyte, resulting in an enhanced output signal. To further lower the detection limits to picomolar levels, nanomaterials have attracted great interest in the design of aptamer-based sensing platforms. Associated to their unique properties, nanomaterials offer great promise for numerous aptasensing applications. This review will discuss current research activities in the aptasensing with typical example of detection of ochratoxin A (OTA). OTA, a secondary fungal metabolite, contaminates a variety of food commodities, and has several toxicological effects such as nephrotoxic, hepatotoxic, neurotoxic, teratogenic and immunotoxic activities. The review will introduce advances made in the methods of integrating nanomaterials in aptasensing, and will discuss current conformational switchable design strategies in aptasensor fabrication methodologies.Keywords
This publication has 96 references indexed in Scilit:
- Immobilization Techniques in the Fabrication of Nanomaterial-Based Electrochemical Biosensors: A ReviewSensors, 2013
- Highly sensitive ochratoxin A impedimetric aptasensor based on the immobilization of azido-aptamer onto electrografted binary film via click chemistryTalanta, 2012
- Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold NanoparticlesSensors, 2012
- Waveguide-Mode Sensors as AptasensorsSensors, 2012
- Revision of ochratoxin a production capacity by the main species of Aspergillus section Circumdati. Aspergillus steynii revealed as the main risk of OTA contaminationFood Control, 2011
- Screening and Initial Binding Assessment of Fumonisin B1 AptamersInternational Journal of Molecular Sciences, 2010
- Switch-based biosensors: a new approach towards real-time, in vivo molecular detectionTrends in Biotechnology, 2010
- Structure-switching biosensors: inspired by NatureCurrent Opinion in Structural Biology, 2010
- Folding-Based Electrochemical Biosensors: The Case for Responsive Nucleic Acid ArchitecturesAccounts of Chemical Research, 2010
- Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and Biological ApplicationsChemical Reviews, 2009