Single-cell label-free photoacoustic flowoxigraphy in vivo
Open Access
- 27 March 2013
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 110 (15), 5759-5764
- https://doi.org/10.1073/pnas.1215578110
Abstract
Label-free functional imaging of single red blood cells (RBCs) in vivo holds the key to uncovering the fundamental mechanism of oxygen metabolism in cells. To this end, we developed single-RBC photoacoustic flowoxigraphy (FOG), which can image oxygen delivery from single flowing RBCs in vivo with millisecond-scale temporal resolution and micrometer-scale spatial resolution. Using intrinsic optical absorption contrast from oxyhemoglobin (HbO2) and deoxyhemoglobin (HbR), FOG allows label-free imaging. Multiple single-RBC functional parameters, including total hemoglobin concentration (CHb), oxygen saturation (sO2), sO2 gradient ( ), flow speed (vf), and oxygen release rate (rO2), have been quantified simultaneously in real time. Working in reflection instead of transmission mode, the system allows minimally invasive imaging at more anatomical sites. We showed the capability to measure relationships among sO2, , vf, and rO2 in a living mouse brain. We also demonstrated that single-RBC oxygen delivery was modulated by changing either the inhalation gas or blood glucose. Furthermore, we showed that the coupling between neural activity and oxygen delivery could be imaged at the single-RBC level in the brain. The single-RBC functional imaging capability of FOG enables numerous biomedical studies and clinical applications.Keywords
This publication has 25 references indexed in Scilit:
- Oxygen modulation of neurovascular coupling in the retinaProceedings of the National Academy of Sciences of the United States of America, 2011
- Functional ultrasound imaging of the brainNature Methods, 2011
- Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vesselsNature Medicine, 2011
- Label-free oxygen-metabolic photoacoustic microscopy in vivoJournal of Biomedical Optics, 2011
- Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissueNature Methods, 2010
- Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networksNature Methods, 2010
- Multiscale photoacoustic microscopy and computed tomographyNature Photonics, 2009
- Functional transcranial brain imaging by optical-resolution photoacoustic microscopyJournal of Biomedical Optics, 2009
- Neurovascular regulation in the normal brain and in Alzheimer's diseaseNature Reviews Neuroscience, 2004
- Diffuse Optical Tomography of Cerebral Blood Flow, Oxygenation, and Metabolism in Rat during Focal IschemiaJournal of Cerebral Blood Flow & Metabolism, 2003