Enzyme levels used to monitor pesticide resistance inVarroa jacobsoni
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Apicultural Research
- Vol. 30 (1), 17-20
- https://doi.org/10.1080/00218839.1991.11101229
Abstract
SUMMARY The contingency of pesticide resistance in the mite Varroa jacobsoni Oudemans prompted this preliminary study, in which methodology for assaying levels of two detoxification systems, namely esterases and glutathione S-transferase (GST), was developed. The total esterolytic activity detected in post- mitochondrial fractions of a homogenized 200-mg sample of V. jacobsoni from 10 colonies was 0.0068 (OD) min−1 μg−1 protein. GST activity, expressed in μmol conjugate min−1 μg−1 protein was 0.260±0.21 in the post-mitochondrial supernatant and 0.646±0.48 in the cytosol. These results pertain only to mites obtained from a single experimental apiary of A. mellifera ligustica in Israel. The methodology and preliminary results obtained may be used for subsequent local or regional monitoring of enzyme levels associated with pesticide resistance in V. jacobsoni.Keywords
This publication has 15 references indexed in Scilit:
- Resistance in Constant Exposure Livestock Insect Control Systems: A Partial Review with Some Original Findings on Cyromazine Resistance in House FliesThe Florida Entomologist, 1989
- A mixed function oxidase factor contributing to permethrin and dichlorvos resistance in Lycoriella mali (fitch) (diptera: Sciaridae)Pesticide Science, 1989
- Comparative metabolism of the pyrethroids bifenthrin and deltamethrin in the bulb mite Rhizoglyphus robiniJournal of Agricultural and Food Chemistry, 1988
- Analysis of methidathion resistance mechanisms in Phytoseiulus persimilis A.HPesticide Biochemistry and Physiology, 1987
- Hydrolysis of permethrin by pyrethroid esterases from resistant and susceptible strains of Amblyseius fallacis (Acari: Phytoseiidae)Pesticide Biochemistry and Physiology, 1986
- Separation and characterization of the pyrethroid-hydrolyzing esterases of the cattle tick, Boophilus microplusPesticide Biochemistry and Physiology, 1985
- Factors affecting resistance to insecticides in house-flies, Musca domestica L. (Diptera: Muscidae). II. Close linkage on autosome 2 between an esterase and resistance to trichlorphon and pyrethroidsBulletin of Entomological Research, 1984
- SELECTION FOR AND GENETIC ANALYSIS OF PERMETHRIN RESISTANCE IN METASEIULUS OCCIDENTALS: GENETIC IMPROVEMENT OF A BIOLOGICAL CONTROL AGENTEntomologia Experimentalis et Applicata, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970