The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential
Open Access
- 10 December 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Applied Microbiology and Biotechnology
- Vol. 93 (1), 331-341
- https://doi.org/10.1007/s00253-011-3713-5
Abstract
Pantoea ananatis AJ13355 is a newly identified member of the Enterobacteriaceae family with promising biotechnological applications. This bacterium is able to grow at an acidic pH and is resistant to saturating concentrations of L-glutamic acid, making this organism a suitable host for the production of L-glutamate. In the current study, the complete genomic sequence of P. ananatis AJ13355 was determined. The genome was found to consist of a single circular chromosome consisting of 4,555,536 bp [DDBJ: AP012032] and a circular plasmid, pEA320, of 321,744 bp [DDBJ: AP012033]. After automated annotation, 4,071 protein-coding sequences were identified in the P. ananatis AJ13355 genome. For 4,025 of these genes, functions were assigned based on homologies to known proteins. A high level of nucleotide sequence identity (99%) was revealed between the genome of P. ananatis AJ13355 and the previously published genome of P. ananatis LMG 20103. Short colinear regions, which are identical to DNA sequences in the Escherichia coli MG1655 chromosome, were found to be widely dispersed along the P. ananatis AJ13355 genome. Conjugal gene transfer from E. coli to P. ananatis, mediated by homologous recombination between short identical sequences, was also experimentally demonstrated. The determination of the genome sequence has paved the way for the directed metabolic engineering of P. ananatis to produce biotechnologically relevant compounds.Keywords
This publication has 30 references indexed in Scilit:
- Genome Sequence of the Biocontrol Agent Pantoea vagans Strain C9-1Journal of Bacteriology, 2010
- Non-coding RNA detection methods combined to improve usability, reproducibility and precisionBMC Bioinformatics, 2010
- progressiveMauve: Multiple Genome Alignment with Gene Gain, Loss and RearrangementPLOS ONE, 2010
- Genome Sequence of Pantoea ananatis LMG20103, the Causative Agent of Eucalyptus Blight and DiebackJournal of Bacteriology, 2010
- Use of the λ Red-recombineering method for genetic engineering of Pantoea ananatisBMC Molecular Biology, 2009
- Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structureBMC Biotechnology, 2008
- A genome‐scale metabolic reconstruction for Escherichia coli K‐12 MG1655 that accounts for 1260 ORFs and thermodynamic informationMolecular Systems Biology, 2007
- Improved microbial gene identification with GLIMMERNucleic Acids Research, 1999
- Phylogenetic Analysis of Erwinia Species Based on 16S rRNA Gene SequencesInternational Journal of Systematic and Evolutionary Microbiology, 1997
- The Complete Genome Sequence of Escherichia coli K-12Science, 1997