Molecular immune signatures of HIV-1 vaccines in human PBMCs
- 6 August 2009
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 583 (18), 3004-3008
- https://doi.org/10.1016/j.febslet.2009.07.060
Abstract
The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.Funding Information
- Ministero Italiano Università e Ricerca (MIUR, 2004)
- the Ministero Italiano della Sanità (Ricerca Corrente and Progetto Finalizzato AIDS 2006)
- Institute of Human Virology and the NIH
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