Protective role for club cell secretory protein-16 (CC16) in the development of COPD
- 19 February 2015
- journal article
- Published by European Respiratory Society (ERS) in European Respiratory Journal
- Vol. 45 (6), 1544-1556
- https://doi.org/10.1183/09031936.00134214
Abstract
Club cell secretory protein-16 (CC16) is the major secreted product of airway club cells, but its role in the pathogenesis of chronic obstructive pulmonary disease (COPD) is unclear. We measured CC16 airway expression in humans with and without COPD and CC16 function in a cigarette smoke (CS)-induced COPD murine model.Airway CC16 expression was measured in COPD patients, smokers without COPD and non-smokers. We exposed wildtype (WT) andCC16−/−mice to CS or air for up to 6 months, and measured airway CC16 expression, pulmonary inflammation, alveolar septal cell apoptosis, airspace enlargement, airway mucin 5AC (MUC5AC) expression, small airway remodelling and pulmonary function.Smokers and COPD patients had reduced airway CC16 immunostaining that decreased with increasing COPD severity. Exposing mice to CS reduced airway CC16 expression.CC16−/−mice had greater CS-induced emphysema, airway remodelling, pulmonary inflammation, alveolar cell apoptosis, airway MUC5AC expression, and more compliant lungs than WT mice. These changes were associated with increased nuclear factor-κB (NF-κB) activation inCC16−/−lungs. CS-induced acute pulmonary changes were reversed by adenoviral-mediated over-expression of CC16.CC16 protects lungs from CS-induced injury by reducing lung NF-κB activation. CS-induced airway CC16 deficiency increases CS-induced pulmonary inflammation and injury and likely contributes to the pathogenesis of COPD.Keywords
Funding Information
- Flight Attendant Medical Research Institute (FAMRI CIA 123046)
- BWH-LRRI Consortium
- NHLBI (P01 HL114501, P50 HL107165-01, R01 AI111475, R21 HL111835)
This publication has 40 references indexed in Scilit:
- The Involvement of Phospholipases A2in Asthma and Chronic Obstructive Pulmonary DiseaseMediators of Inflammation, 2013
- Clara Cell 10-kDa Protein Gene Transfection Inhibits NF-κB Activity in Airway Epithelial CellsPLOS ONE, 2012
- IκB kinase–driven nuclear factor-κB activation in patients with asthma and chronic obstructive pulmonary diseaseJournal of Allergy and Clinical Immunology, 2011
- Clara Cells Attenuate the Inflammatory Response through Regulation of Macrophage BehaviorAmerican Journal of Respiratory Cell and Molecular Biology, 2010
- Distal Airways in Mice Exposed to Cigarette SmokeAmerican Journal of Respiratory Cell and Molecular Biology, 2008
- Effect of an MMP-9/MMP-12 inhibitor on smoke-induced emphysema and airway remodelling in guinea pigsThorax, 2007
- The Association between the Anti-inflammatory Protein CC10 and Smoking Status among Participants in a Chemoprevention TrialCancer Epidemiology, Biomarkers & Prevention, 2007
- A Two-receptor Pathway for Catabolism of Clara Cell Secretory Protein in the KidneyJournal of Biological Chemistry, 2001
- Dose-Dependent Tolerance to Ozone: III. Elevation of Intracellular Clara Cell 10-kDa Protein in Central Acini of Rats Exposed for 20 MonthsToxicology and Applied Pharmacology, 1994
- Immunohistochemical Demonstration of Cytochrome P-450 Monooxygenase in Clara Cells throughout the Tracheobronchial Airways of the RabbitExperimental Lung Research, 1987