Expression Pattern of Claudins 5 and 7 Distinguishes Solid-pseudopapillary From Pancreatoblastoma, Acinar Cell and Endocrine Tumors of the Pancreas
- 1 May 2009
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in The American Journal of Surgical Pathology
- Vol. 33 (5), 768-774
- https://doi.org/10.1097/pas.0b013e3181957bc4
Abstract
Solid-pseudopapillary tumor (SPT) of the pancreas is characterized by a discohesive appearance of the neoplastic cells. This has been linked to the displacement of E-cadherin and β-catenin from their normal membrane location, which prevents adherens junctions to form. The nuclear localization of β-catenin is also a feature of SPT that helps in differential diagnosis. This latter includes pancreatic endocrine tumor (PET) as SPT may show neuroendocrine differentiation, and pancreatic acinar cell carcinoma (ACC) and pancreatoblastoma (PB) that may often show nuclear β-catenin staining. However, the role of additional cell-cell adhesion systems remains to be elucidated in SPT, particularly that of claudins that are essential components of tight junctions showing modulated expression in diverse tumor types. We studied 20 SPT, 20 nonfunctioning PET, 7 ACC, 2 PB, and their matched normal pancreas for the immunohistochemical expression of claudin family members 1, 2, 3, 4, 5, and 7, β-catenin and E-cadherin. All SPT showed intense membrane claudin 5 and cytoplasmic claudin 2 staining, lack of claudins 3 and 4, and positive cytoplasmic claudins 1 and 7 in few cases. Conversely, PET, ACC, and PB showed strong membrane expression of claudin 7 and lack of claudin 5, whereas claudins 1, 2, 3, and 4 showed variable expression among samples. All SPT showed nuclear β-catenin and lack of E-cadherin membrane staining, whereas PET, ACC, and PB only showed nuclear β-catenin in 1, 2, and 2 cases, respectively. SPT shows a peculiar claudin expression profile and the highly specific pattern of claudins 5 and 7 differentiates SPT from PET, ACC, and PB.Keywords
This publication has 27 references indexed in Scilit:
- Membrane loss and aberrant nuclear localization of E‐cadherin are consistent features of solid pseudopapillary tumour of the pancreas. An immunohistochemical study using two antibodies recognizing different domains of the E‐cadherin moleculeHistopathology, 2008
- E-cadherin/Catenin Complex Status in Solid Pseudopapillary Tumor of the PancreasThe American Journal of Surgical Pathology, 2008
- Claudin expression in pancreatic endocrine tumors as compared with ductal adenocarcinomasVirchows Archiv, 2007
- Radiolabeled anti-claudin 4 and anti-prostate stem cell antigen: initial imaging in experimental models of pancreatic cancer.Molecular Imaging, 2007
- Claudin-1 regulates cellular transformation and metastatic behavior in colon cancerJCI Insight, 2005
- Expression of novel markers of pancreatic ductal adenocarcinoma in pancreatic nonductal neoplasms: additional evidence of different genetic pathwaysLaboratory Investigation, 2005
- Solid-Pseudopapillary Tumors of the Pancreas Are Genetically Distinct from Pancreatic Ductal Adenocarcinomas and Almost Always Harbor β-catenin MutationsThe American Journal of Pathology, 2002
- Genetic and Immunohistochemical Analysis of Pancreatic Acinar Cell Carcinoma: Frequent Allelic Loss on Chromosome 11p and Alterations in the APC/β-Catenin PathwayThe American Journal of Pathology, 2002
- Distinctive Molecular Genetic Alterations in Sporadic and Familial Adenomatous Polyposis-Associated PancreatoblastomasThe American Journal of Pathology, 2001
- Solid and Papillary Tumor of the Pancreas: Ultrastructural Observations on Two Contrasting CasesUltrastructural Pathology, 1997