Effects of genetic ablation of bach1 upon smooth muscle cell proliferation and atherosclerosis after cuff injury

Abstract

Bach1 is a transcriptional repressor of the cytoprotective enzyme heme oxygenase-1 (HO-1). Although HO-1 protects against atherosclerosis, the function of Bach1 in this process is poorly understood. We isolated peri- toneal macrophages and aortic smooth muscle cells (SMC) from wild-type and bach1 -deficient mice. bach1 - deficient macrophages expressed increased levels of HO-1 and showed elevated phagocytic activity when incubated with 0.75 µ µ µ µ m microspheres. In SMC, bach1 -ablation resulted in increased expression of HO-1 and decreased proliferation in bromodeoxyuridine incorporation assay as compared with wild-type cells. The up-regulated phagocytic activity and reduced SMC proliferation of bach1 -deficient cells were not restored by Zinc (II) protoporphyrin IX, an inhibitor of HO, suggesting that HO-independent mechanisms are also involved in the regulation of phagocytosis of macrophages and proliferation of SMC by Bach1. In wild-type mice, cuff placement around femoral artery caused pronounced intimal proliferation without affecting the media, thus resulting in intimal to medial (I/M) volume ratio of 65.6%. bach1 -deficient mice had less degree of intimal growth (I/M ratio of 45.6%). These results indicate that Bach1 plays a critical role in the regulation of HO-1 expression, macrophage function, SMC proliferation and neointimal formation. Bach1 may regu- late gene expression in these cells during inflammation and atherogenesis.