Evidence for regular distribution of sterols in liquid crystalline phosphatidylcholine bilayers.
- 11 October 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 91 (21), 10069-10073
- https://doi.org/10.1073/pnas.91.21.10069
Abstract
To investigate the lateral organization of sterols in membranes, the fluorescence intensity of dehydroergosterol at different mole fractions in liquid crystalline dimyristoyl phosphatidylcholine bilayers was examined. A number of intensity drops were observed at specific mole fractions, as predicted from a hexagonal super-lattice model. The fluorescence dips provide compelling evidence that a naturally occurring sterol is regularly distributed at fixed compositional fractions, consistent with the presence of hexagonal super-lattices in the fluid membranes. Regularly distributed regions, however, coexist with irregularly distributed regions. The extent of regular distribution varies periodically with sterol mole fraction and, consequently, similar variations take place in the membrane volume and lipid packing. This level of modulation in local membrane structure by minute changes in sterol concentration should have profound implications for the functional role of cholesterol content in cell membranes.Keywords
This publication has 19 references indexed in Scilit:
- Sterol domains in phospholipid membranes: dehydroergosterol polarization measures molecular sterol transferJournal of Biochemical and Biophysical Methods, 1992
- Lateral diffusion in the liquid phases of dimyristoylphosphatidylcholine/cholesterol lipid bilayers: a free volume analysisBiochemistry, 1992
- A fluorescence study of dehydroergosterol in phosphatidylcholine bilayer vesiclesBiochemistry, 1987
- 1-Palmitoyl-2-pyrenedecanoyl glycerophospholipids as membrane probes: evidence for regular distribution in liquid-crystalline phosphatidylcholine bilayersBiochemistry, 1985
- Incorporation of dansylated phospholipids and dehydroergosterol into membranes using a phospholipid exchange proteinBiochemistry, 1983
- Dilatometry of dipalmitoyllecithin-cholesterol bilayersBiochemistry, 1980
- Cholesterol-phosphatidylcholine interactions in multilamellar vesiclesBiochemistry, 1980
- The organisation of cholesterol and ergosterol in lipid bilayers based on studies using non-perturbing fluorescent sterol probesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- Estimation of lateral species separation from phase transitions in nonideal two-dimensional lipid mixturesBiochemistry, 1978
- Nuclear Magnetic Resonance Spectroscopic Studies of the Interaction of Phospholipids with CholesterolNature, 1966