p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway
Open Access
- 22 July 2009
- journal article
- research article
- Published by Hindawi Limited in Journal of Biomedicine and Biotechnology
- Vol. 2009, 1-11
- https://doi.org/10.1155/2009/181282
Abstract
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,-DDE-induced toxicity in male reproductive system. The results indicated that p,-DDE exposure at over 30 M showed the induction of apoptotic cell death. p,-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,-DDE treatment in these cells. The activation of NF-B was enhanced with the increase of p,-DDE dose. Taken together, these results suggested that exposure to p,-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.
Keywords
Funding Information
- National Natural Science Foundation of China (40590390, 30671734)
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