Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
Open Access
- 1 November 2009
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 90 (11), 2669-2678
- https://doi.org/10.1099/vir.0.014183-0
Abstract
The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known ‘insect-only’ flavivirus, appearing to replicate in insect cells alone. A second member of the ‘insect-only’ group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the ‘insect-only’ group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel ‘insect-only’ flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa.Keywords
This publication has 42 references indexed in Scilit:
- Flavivirus RNA in Phlebotomine SandfliesVector-Borne and Zoonotic Diseases, 2010
- BEAST: Bayesian evolutionary analysis by sampling treesBMC Evolutionary Biology, 2007
- Improvement of Phylogenies after Removing Divergent and Ambiguously Aligned Blocks from Protein Sequence AlignmentsSystematic Biology, 2007
- bold: The Barcode of Life Data System (http://www.barcodinglife.org)Molecular Ecology Notes, 2007
- LoPPS: A long PCR product sequencing method for rapid characterisation of long ampliconsBiochemical and Biophysical Research Communications, 2006
- Phylogeny and classification of Aedini (Diptera: Culicidae), based on morphological characters of all life stagesZoological Journal of the Linnean Society, 2004
- MUSCLE: multiple sequence alignment with high accuracy and high throughputNucleic Acids Research, 2004
- Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virusArchiv für die gesamte Virusforschung, 2003
- Isolation of a new flavivirus related to Cell fusing agent virus (CFAV) from field-collected flood-water Aedes mosquitoes sampled from a dambo in central KenyaArchiv für die gesamte Virusforschung, 2003
- Biological identifications through DNA barcodesProceedings Of The Royal Society B-Biological Sciences, 2003