Insights into the Soluble PEG-Supported Synthesis of Cytosine-Containing Nucleoside 5′-Mono-, Di-, and Triphosphates
- 9 November 2009
- journal article
- Published by American Chemical Society (ACS) in The Journal of Organic Chemistry
- Vol. 74 (23), 9165-9172
- https://doi.org/10.1021/jo901931z
Abstract
Nucleoside phosphoesters (nucleotides) have crucial roles in a large variety of biological processes including nucleic acid biosynthesis and their corresponding analogues are extensively used as biological tools. Herein, we describe a new and efficient synthetic procedure involving polyethylene glycol (PEG) as soluble support and regioselective mono-, di-, and triphosphorylation steps. Applied to natural and synthetic cytosine containing nucleosides, this methodology allowed the preparation of various phosphorylated forms in high yields and good purity.Keywords
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