Decellularized Porcine Brain Matrix for Cell Culture and Tissue Engineering Scaffolds
- 1 November 2011
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part A
- Vol. 17 (21-22), 2583-2592
- https://doi.org/10.1089/ten.tea.2010.0724
Abstract
The extracellular matrix (ECM) plays important roles in influencing cellular behavior such as attachment, differentiation, and proliferation. However, in conventional culture and tissue engineering strategies, single proteins are frequently utilized, which do not mimic the complex extracellular microenvironment seen in vivo. In this study we report a method to decellularize brain tissue using detergents. This decellularized brain matrix is rich in glycosaminoglycans and contains collagen I, collagen III, collagen IV, collagen V, collagen VI, perlecan, and laminin. By further processing the material into a liquid form, the brain matrix can be used as a cell culture coating. Neurons derived from human induced pluripotent stem cells plated on the brain matrix express neuronal markers and assume neuronal morphology. Additionally, the same material can potentially be used as a scaffold for tissue engineering as it reassembles upon injection in vivo to form a gel. Thus, our work demonstrates the ability to use decellularized brain ECM for cell culture and tissue engineering applications.Keywords
This publication has 55 references indexed in Scilit:
- Injectable hydrogel scaffold from decellularized human lipoaspirateActa Biomaterialia, 2011
- Design and Characterization of an Injectable Pericardial Matrix Gel: A Potentially Autologous Scaffold for Cardiac Tissue EngineeringTissue Engineering, Part A, 2010
- Extraction and Assembly of Tissue-Derived Gels for Cell Culture and Tissue EngineeringTissue Engineering, Part C: Methods, 2009
- Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineeringBiomaterials, 2009
- Sex differences in the dendritic arbor of hypothalamic ventromedial nucleus neuronsPhysiology & Behavior, 2009
- TOX3 regulates calcium-dependent transcription in neuronsProceedings of the National Academy of Sciences of the United States of America, 2009
- Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cellsBMC Developmental Biology, 2008
- Quantification of DNA in Biologic Scaffold MaterialsJournal of Surgical Research, 2008
- Fibronectin Expression Modulates Mammary Epithelial Cell Proliferation during Acinar DifferentiationCancer Research, 2008
- Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cellsProceedings of the National Academy of Sciences of the United States of America, 2006