Transforming growth factor ?1-induced cellular heterogeneity in the periosteum of rat parietal bones
- 1 August 1993
- journal article
- research article
- Published by Springer Science and Business Media LLC in Calcified Tissue International
- Vol. 53 (2), 122-126
- https://doi.org/10.1007/bf01321890
Abstract
We examined the osteogenesis process in transforming growth factor β1 (TGF-β1)-treated neonatal and adult rats, aiming to investigate the age difference in the effect of TGF-β1 on mesenchymal cell differentiation. Recombinant human (rh) TGF-β1 (20 and 200 ng) was injected onto the outer periostea of the right side of the parietal bone of each rat once a day for 1–12 days starting at the age of either 1 day or 12 weeks. On the day after the final injection, the calvaria was excised and evaluated histologically. In the neonates, the 12-day treatment with rhTGF-β1 increased the number of osteoprogenitor cells, resulting in intramembranous ossification. In the adult rats, rhTGF-β1 induced differentiation of chondrocytes. Cartilage masses were surrounded by mesenchymal cells, which would differentiate into chondrocytes. The cartilage matrix was partially calcified, with chondrocytes buried therein. In the calcified matrix, marrow cavities containing some multinuclear osteoclasts were formed. These findings indicate that rhTGF-β1 stimulated the differentiation of mesenchymal cells into chondrocytes and produced the cartilaginous matrix. rhTGF-β1 induced intramembranous ossification of the parietal bone in neonatal rats, and it induced enchondral ossification in adults. This result suggests that the different responses of mesenchymal cells in the periosteum to rhTGF-β1 may depend on the age of the animals used: namely, they may reflect the respective osteogenic stages of modeling and remodeling.Keywords
This publication has 27 references indexed in Scilit:
- Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitroJournal of Bone and Mineral Research, 1991
- Parathyroid hormone and transforming growth factor β synergistically stimulate formation of prostaglandin E2 in neonatal mouse calvarial bonesActa Physiologica Scandinavica, 1991
- In vivo induction of bone by recombinant human transforming growth factor β1Journal of Bone and Mineral Research, 1991
- Stimulation of chondrogenesis in limb bud mesoderm cells by recombinant human bone morphogenetic protein 2B (BMP-2B) and modulation by transforming growth factor ?1 and ?2*1Experimental Cell Research, 1991
- In vivo effects of human recombinant transforming growth factor β on bone turnover in normal miceJournal of Bone and Mineral Research, 1990
- Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur.The Journal of cell biology, 1990
- Accumulation, localization, and compartmentation of transforming growth factor beta during endochondral bone development.The Journal of cell biology, 1988
- Effect of transforming growth factor β on cell proliferation and glycosaminoglycan synthesis by rabbit growth-plate chondrocytes in cultureBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1988
- Earliest Evidence of Cartilage and Bone Development in Embryonic LifeClinical Orthopaedics and Related Research, 1987
- Differentiation of rat mesenchymal cells by cartilage-inducing factorExperimental Cell Research, 1986