Large-Scale Gene Discovery in the OomycetePhytophthora infestansReveals Likely Components of Phytopathogenicity Shared with True Fungi
Open Access
- 1 March 2005
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 18 (3), 229-243
- https://doi.org/10.1094/mpmi-18-0229
Abstract
To overview the gene content of the important pathogen Phytophthora infestans, large-scale cDNA and genomic sequencing was performed. A set of 75,757 high-quality expressed sequence tags (ESTs) from P. infestans was obtained from 20 cDNA libraries representing a broad range of growth conditions, stress responses, and developmental stages. These included libraries from P. infestans-potato and -tomato interactions, from which 963 pathogen ESTs were identified. To complement the ESTs, onefold coveragethe P. infestans genome was obtained and regions of coding potential identified. A unigene set of 18,256 sequences was derived from the EST and genomic data and characterized for potential functions, stage-specific patterns of expression, and codon bias. Cluster analysis of ESTs revealed major differences between the expressed gene content of mycelial and spore-related stages, and affinities between some growth conditions. Comparisons with databases of fungal pathogenicity genes revealed conserved elements of pathogenicity, such as class III pectate lyases, despite the considerable evolutionary distance between oomycetes and fungi. Thirty-seven genes encoding components of flagella also were identified. Several genes not anticipated to occur in oomycetes were detected, including chitin synthases, phosphagen kinases, and a bacterial-type FtsZ cell-division protein. The sequence data described are available in a searchable public database.Keywords
This publication has 78 references indexed in Scilit:
- The hAT -like DNA transposon DodoPi resides in a cluster of retro- and DNA transposons in the stramenopile Phytophthora infestansMolecular Genetics and Genomics, 2004
- Diverse Eukaryotes have Retained Mitochondrial Homologues of the Bacterial Division Protein FtsZProtist, 2004
- The genome sequence of the filamentous fungus Neurospora crassaNature, 2003
- Comparative Analyses of Potato Expressed Sequence Tag LibrariesPlant Physiology, 2003
- Phytophthora ramorum sp. nov., a new pathogen on Rhododendron and ViburnumMycological Research, 2001
- Physical mapping across an avirulence locus of Phytophthora infestans using a highly representative, large-insert bacterial artificial chromosome libraryMolecular Genetics and Genomics, 2001
- Initial Assessment of Gene Diversity for the Oomycete Pathogen Phytophthora infestans Based on Expressed SequencesFungal Genetics and Biology, 1999
- Fungi, flagella and phylogenyMycological Research, 1997
- Basic local alignment search toolJournal of Molecular Biology, 1990
- The ‘effective number of codons’ used in a geneGene, 1990