Synthesis of chitosan biocomposites loaded with pyrrole-2-carboxylic acid and assessment of their antifungal activity against Aspergillus niger
- 12 February 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Applied Microbiology and Biotechnology
- Vol. 103 (7), 2985-3000
- https://doi.org/10.1007/s00253-019-09670-w
Abstract
A wide variety of chitosan (CS) biomaterials have been loaded with different antimicrobial agents to improve the activity of CS against phytopathogenic fungi. Recently, the antimicrobial activity of 1H-pyrrole-2-carboxylic acid (PCA) has been reported as a secondary metabolite of Streptomyces griseus, which was identified as the main bioactive compound in the biological control. However, it is sensitive to light and its activity against filamentous fungi has not yet been reported. The aim of the present research work was to evaluate the biological activity of CS-PCA biocomposites for the control of Aspergillus niger. CS-PCA biocomposites were obtained through nanoprecipitation. In vitro antifungal activity was determined by viability assay, spore germination, morphometric analysis of spores and hyphae, and the analysis of cellular components by fluorescence microscopy. CS-PCA showed an average size and Z potential of 502 ± 72 nm and + 54.7 ± 15 mV, respectively. Micrographs demonstrated well-distributed biocomposites with an apparently spherical shape. A new signal at 1473 cm−1 in the FT-IR spectrum of the CS-PCA biocomposite was observed, confirming the presence of PCA in the composition of the CS-PCA nanosystem. CS-PCA biocomposites reduced the spores’ viability by up to 58%. Effects on fungi morphometry, observed as an increase in the spores’ average diameter, swelling, distortion, and an increase in the branching of hyphae, were observed. Fluorescence analysis showed oxidative stress and membrane and cell wall damage, mainly at early growth stages. The inhibitory effect against CS-resistant fungi, such as A. niger, opens a door for the control of CS-sensitive fungi.Keywords
Funding Information
- Consejo Nacional de Ciencia y Tecnolog?a (project 219786, scholarship)
This publication has 52 references indexed in Scilit:
- Antifungal Activity of Chitosan Nanoparticles and Correlation with Their Physical PropertiesInternational Journal of Biomaterials, 2012
- How does a hypha grow? The biophysics of pressurized growth in fungiNature Reviews Microbiology, 2011
- Antifungal effect of chitosan on the growth of Aspergillus parasiticus and production of aflatoxin B1Polymer International, 2011
- Antifungal activity of films and solutions based on chitosan against typical seed fungiFood Hydrocolloids, 2009
- Antagonistic Effects of Streptomyces sp. SRM1 on Colletotrichum musaeBiotechnology, 2008
- Antibacterial characteristics and activity of acid-soluble chitosanBioresource Technology, 2008
- A protocol for in vivo detection of reactive oxygen speciesProtocol Exchange, 2008
- Development and characterization of biodegradable nanospheres as delivery systems of anti-ischemic adenosine derivativesBiomaterials, 2005
- Effect of Chitosan and Temperature on Spore Germination of Aspergillus nigerMacromolecular Bioscience, 2003
- A Simplified New Assay for Assessment of Fungal Cell Damage with the Tetrazolium Dye, (2,3)-bis-(2-Methoxy-4-Nitro-5-Sulphenyl)-(2H)-Tetrazolium-5-Carboxanilide (XTT)The Journal of Infectious Diseases, 1995