Lactose-induced Production of Human Soluble B Lymphocyte Stimulator (hsBLyS) in E. coli with Different Culture Strategies
- 1 April 2006
- journal article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 28 (7), 477-483
- https://doi.org/10.1007/s10529-006-0002-y
Abstract
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l−1 and a productivity of 0.11 g l−1 h−1.Keywords
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