Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

Abstract

Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique. Access the supplementary material to this article: Refractive index estimation (see Supplementary files under Article Tools online).