Flow Cytometric Measurement of Hemoglobin F in RBCs
Open Access
- 1 June 2002
- journal article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 117 (6), 857-863
- https://doi.org/10.1309/a63x-hg9t-vyg2-x6tx
Abstract
The cellular distribution of hemoglobin F is important for evaluating persistently elevated hemoglobin F levels, such as in hereditary persistence of fetal hemoglobin (HPFH) or delta/beta-thalassemia, and for differentiating homozygous hemoglobin S (or hemoglobin S–beta0-thalassemia) from hemoglobin S–HPFH, traditionally done by using the Kleihauer-Betke (K-B) acid elution test. We evaluated a flow cytometric method using an anti–hemoglobin F antibody as a replacement for the K-B test. We used 172 specimens representing a variety of conditions: HPFH trait, 19 cases; delta/beta-thalassemia trait, 8 cases; hemoglobin S–HPFH, 10 cases. By flow cytometry, all cases of HPFH trait gave a hemoglobin F pattern comparable to the homocellular pattern obtained by the K-B test; all cases of delta/beta-thalassemia tested gave a pattern comparable to a K-B heterocellular pattern. Most cases of hemoglobin S–HPFH gave a homocellular distribution of hemoglobin F, whereas all cases of homozygous hemoglobin S with elevated hemoglobin F levels gave a heterocellular pattern. Flow cytometry provides a more rapid and objective method for assessing cellular distribution of hemoglobin F and is useful for patient evaluation when HPFH trait, delta/beta-thalassemia trait, or hemoglobin S–HPFH trait is suspected.Keywords
This publication has 9 references indexed in Scilit:
- Detection of fetal red cells in fetomaternal hemorrhage using a fetal hemoglobin monoclonal antibody by flow cytometryTransfusion, 1998
- δβ Thalassemia and Hereditary Persistence of Fetal HemoglobinHematology/Oncology Clinics of North America, 1991
- The deletion in both common types of hereditary persistence of fetal hemoglobin is approximately 105 kilobasesBlood, 1987
- G gamma beta+ hereditary persistence of fetal hemoglobin: cosmid cloning and identification of a specific mutation 5' to the G gamma gene.Proceedings of the National Academy of Sciences of the United States of America, 1984
- Sickle Cell Trait/Hereditary Persistence of Fetal Hemoglobin TraitAmerican Journal of Diseases of Children, 1979
- The Negro Variety of Hereditary Persistence of Fetal Haemoglobin is a Mild Form of ThalassaemiaBritish Journal of Haematology, 1976
- A new form of hereditary persistence of fetal hemoglobin in blacks and its association with sickle cell traitBlood, 1975
- Hereditary Persistence of Foetal Haemoglobin Production, and its Interaction with the Sickle‐Cell TraitBritish Journal of Haematology, 1958
- Demonstration von fetalem H moglobin in den Erythrocyten eines BlutausstrichsKlinische Wochenschrift, 1957