Characterization and identification of PARM-1 as a new potential oncogene
Open Access
- 31 July 2013
- journal article
- Published by Springer Science and Business Media LLC in Molecular Cancer
- Vol. 12 (1), 84
- https://doi.org/10.1186/1476-4598-12-84
Abstract
The Graffi murine retrovirus is a powerful tool to find leukemia associated oncogenes. Using DNA microarrays, we recently identified several genes specifically deregulated in T- and B-leukemias induced by this virus. In the present study, probsets associated with T-CD8+ leukemias were analyzed and we validated the expression profile of the Parm-1 gene. PARM-1 is a member of the mucin family. We showed that human PARM-1 is an intact secreted protein accumulating predominantly, such as murine PARM-1, at the Golgi and in the early and late endosomes. PARM-1 colocalization with α-tubulin suggests that its trafficking within the cell involves the microtubule cytoskeleton. Also, the protein co-localizes with caveolin-1 which probably mediates its internalization. Transient transfection of both mouse and human Parm-1 cDNAs conferred anchorage- and serum-independent growth and enhanced cell proliferation. Moreover, deletion mutants of human PARM-1 without either extracellular or cytoplasmic portions seem to retain the ability to induce anchorage-independent growth of NIH/3T3 cells. In addition, PARM-1 increases ERK1/2, but more importantly AKT and STAT3 phosphorylation. Our results strongly suggest the oncogenic potential of PARM-1.Keywords
This publication has 46 references indexed in Scilit:
- Loci affecting gamma-glutamyl transferase in adults and adolescents show age × SNP interaction and cardiometabolic disease associationsHuman Molecular Genetics, 2011
- Genetically Abnormal Circulating Cells in Lung Cancer Patients: An Antigen-Independent Fluorescence In situ Hybridization–Based Case-Control StudyClinical Cancer Research, 2010
- HIV-1 Rev–binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in miceJCI Insight, 2010
- Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathwayBlood, 2010
- Mucins in cancer: function, prognosis and therapyNature Reviews Cancer, 2009
- Recurrent translocations involving the IRF4 oncogene locus in peripheral T-cell lymphomasLeukemia, 2008
- Loss of TLE1 and TLE4 from the del(9q) commonly deleted region in AML cooperates with AML1-ETO to affect myeloid cell proliferation and survivalBlood, 2008
- Crosstalk between cancer and immune cells: role of STAT3 in the tumour microenvironmentNature Reviews Immunology, 2007
- Mucin 2 (MUC2) and mucin 5 (MUC5) expression is not associated with prognosis in patients with radically resected ampullary carcinomaJournal of Clinical Pathology, 2006
- Novel Insights into the Pathogenesis of the Graffi Murine Leukemia RetrovirusJournal of Virology, 2006