Diethyldithiocarbamate inhibits induction of macrophage NO synthase
Open Access
- 26 April 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 321 (2-3), 215-218
- https://doi.org/10.1016/0014-5793(93)80111-7
Abstract
We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow‐derived macrophages. A short exposure (between 1 and 16 h) of L929‐cell medium‐preconditioned macrophages to E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS.Keywords
This publication has 14 references indexed in Scilit:
- Cytokines, endotoxin, and glucocorticoids regulate the expression of inducible nitric oxide synthase in hepatocytes.Proceedings of the National Academy of Sciences of the United States of America, 1993
- NO accounts completely for the oxygenated nitrogen species generated by enzymic l-arginine oxygenationBiochemical Journal, 1992
- Induction of nitric oxide synthase in L929 cells by tumour-necrosis factor α is prevented by inhibitors of poly(ADP-ribose) polymeraseBiochemical Journal, 1992
- Quantification of nitric oxide in biological samples by electron spin resonance spectroscopyNeuroprotocols, 1992
- Nitric oxide synthase is a cytochrome P-450 type hemoproteinBiochemistry, 1992
- Dithiocarbamates as potent inhibitors of nuclear factor kappa B activation in intact cells.The Journal of Experimental Medicine, 1992
- Cloning and Characterization of Inducible Nitric Oxide Synthase from Mouse MacrophagesScience, 1992
- L-arginine-derived nitric oxide and the cell-mediated immune responseResearch in Immunology, 1991
- Induction of Tumor Cytotoxicity in Murine Bone Marrow-Derived Macrophages by Two Synthetic Lipopeptide AnaloguesBiological Chemistry Hoppe-Seyler, 1989
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987