Stabilization of β-Catenin by Genetic Defects in Melanoma Cell Lines
- 21 March 1997
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 275 (5307), 1790-1792
- https://doi.org/10.1126/science.275.5307.1790
Abstract
Signal transduction by β-catenin involves its posttranslational stabilization and downstream coupling to the Lef and Tcf transcription factors. Abnormally high amounts of β-catenin were detected in 7 of 26 human melanoma cell lines. Unusual messenger RNA splicing and missense mutations in the β-catenin gene (CTNNB1) that result in stabilization of the protein were identified in six of the lines, and the adenomatous polyposis coli tumor suppressor protein (APC) was altered or missing in two others. In the APC-deficient cells, ectopic expression of wild-type APC eliminated the excess β-catenin. Cells with stabilized β-catenin contained a constitutive β-catenin-Lef-1 complex. Thus, genetic defects that result in up-regulation of β-catenin may play a role in melanoma progression.This publication has 17 references indexed in Scilit:
- Nuclear localization of β-catenin by interaction with transcription factor LEF-1Mechanisms of Development, 1996
- XTcf-3 Transcription Factor Mediates β-Catenin-Induced Axis Formation in Xenopus EmbryosCell, 1996
- Functional interaction of β-catenin with the transcription factor LEF-1Nature, 1996
- The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3.Genes & Development, 1996
- The Nuclear Localization Signal of Lymphoid Enhancer Factor-1 Is Recognized by Two Differentially Expressed Srp1-Nuclear Localization Sequence Receptor ProteinsPublished by Elsevier BV ,1996
- Cell adhesion and signal transduction: the Armadillo connectionTrends in Cell Biology, 1995
- Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling.The Journal of cell biology, 1995
- Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.The Journal of cell biology, 1993
- Association of the APC Gene Product with β-CateninScience, 1993
- Expression of the int-1 gene in transgenic mice is associated with mammary gland hyperplasia and adenocarcinomas in male and female miceCell, 1988