PsTRXh1 and PsTRXh2 Are Both Pea h-Type Thioredoxins with Antagonistic Behavior in Redox Imbalances
- 10 November 2006
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 143 (1), 300-311
- https://doi.org/10.1104/pp.106.089524
Abstract
Thioredoxins (TRXs) are small ubiquitous oxidoreductases involved in disulfide bond reduction of a large panel of target proteins. The most complex cluster in the family of plant TRXs is formed by h-type TRXs. In Arabidopsis (Arabidopsis thaliana), nine members of this subgroup were described, which are less well known than their plastidial counterparts. The functional study of type-h TRXs is difficult because of the high number of isoforms and their similar biochemical characteristics, thus raising the question whether they have specific or redundant functions. Type-h TRXs are involved in seed germination and self incompatibility in pollen-pistil interaction. Their function as antioxidants has recently been proposed, but further work is needed to clarify this function in plants. In this study, we describe two new h-type TRXs from pea (Pisum sativum; stated PsTRXh1 and PsTRXh2). By functional complementation of a yeast (Saccharomyces cerevisiae) trx1Δ trx2Δ double mutant, we demonstrate that PsTRXh1 is involved in the redox-imbalance control, possibly through its interaction with peroxiredoxins. In contrast, PsTRXh2 provokes a phenotype of hypersensitivity to hydrogen peroxide in the yeast mutant. Furthermore, we show differential gene expression and protein accumulation of the two isoforms, PsTRXh1 protein being abundantly detected in vascular tissue and flowers, whereas PsTRXh2 gene expression was hardly detectable. By comparison with previous data of additional PsTRXh isoforms, our results indicate specific functions for the pea h-type TRXs so far described.Keywords
This publication has 65 references indexed in Scilit:
- Cloning and characterization of three thioredoxin h isoforms from wheat showing differential expression in seedsJournal of Experimental Botany, 2006
- The role of stigma peroxidases in flowering plants: insights from further characterization of a stigma-specific peroxidase (SSP) from Senecio squalidus (Asteraceae)Journal of Experimental Botany, 2006
- The thioredoxin h system of higher plantsPlant Physiology and Biochemistry, 2004
- The multigenic family of thioredoxin h in Arabidopsis thaliana: specific expression and stress responsePlant Physiology and Biochemistry, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Purification and Characterization of a Second Type Thioredoxin Peroxidase (Type II TPx) from Saccharomyces cerevisiaeBiochemistry, 1998
- Inhibition of Metal-Catalyzed Oxidation Systems by a Yeast Protector Protein in the Presence of ThioredoxinBiochemical and Biophysical Research Communications, 1994
- Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAsThe Plant Journal, 1992
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970