Optimization and Validation of a GC–FID Method for the Determination of Acetone-Butanol-Ethanol Fermentation Products
Open Access
- 25 March 2013
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 52 (3), 264-270
- https://doi.org/10.1093/chromsci/bmt022
Abstract
An improved, simple gas chromatography–flame ionization detection (GC–FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC–FID (more than 20 min). The behavior of the compounds in temperature-programmed gas chromatographic runs was predicted using thermodynamic parameters derived from isothermal runs. The optimum temperature programming condition was achieved when the resolution for each peak met the analytical requirement and the analysis time was shortest. With the exception of acetic acid, the detection limits of the presented method for various products were below 10 mg/L. The repeatability and intermediate precision of the method were less than 10% (relative standard deviation). Validation and quantification results demonstrated that this method is a sensitive, reliable and fast alternative for conventional investigation of the adsorption-coupled ABE fermentation process.Keywords
This publication has 17 references indexed in Scilit:
- Bio-butanol vs. bio-ethanol: A technical and economic assessment for corn and switchgrass fermented by yeast or Clostridium acetobutylicumBiomass and Bioenergy, 2010
- Metabolic engineering of Escherichia coli for 1-butanol productionMetabolic Engineering, 2008
- Butanol production by Clostridium beijerinckii. Part I: Use of acid and enzyme hydrolyzed corn fiberBioresource Technology, 2008
- Improvements of GC and HPLC analyses in solvent (acetone-butanol-ethanol) fermentation byClostridium saccharobutylicum using a mixture of starch and glycerol as carbon sourceBiotechnology and Bioprocess Engineering, 2006
- Resolution prediction and optimization of temperature programme in comprehensive two-dimensional gas chromatographyJournal of Chromatography A, 2005
- High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-Stat continuous butyric acid and glucose feeding methodJournal of Bioscience and Bioengineering, 2004
- Optimization of temperature-programmed gas chromatographic separations I. Prediction of retention times and peak widths from retention indicesJournal of Chromatography A, 1995
- Improved acetone-butanol fermentation analysis using subambient HPLC column temperatureEnzyme and Microbial Technology, 1990
- Agitation and pressure effects on acetone‐butanol fermentationBiotechnology & Bioengineering, 1985
- Butanol production of Clostridium acetobutylicum grown on sugars found in hemicellulose hydrolysatesBiotechnology Letters, 1982