Novel Three-Dimensional Organoid Model for Evaluation of the Interaction of Uropathogenic Escherichia coli with Terminally Differentiated Human Urothelial Cells
Open Access
- 1 January 2006
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 74 (1), 750-757
- https://doi.org/10.1128/iai.74.1.750-757.2006
Abstract
Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced α-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.Keywords
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