Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells
- 1 March 2002
- journal article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 282 (3), C606-C616
- https://doi.org/10.1152/ajpcell.00269.2001
Abstract
The tensegrity hypothesis holds that the cytoskeleton is a structure whose shape is stabilized predominantly by the tensile stresses borne by filamentous structures. Accordingly, cell stiffness must increase in proportion with the level of the tensile stress, which is called the prestress. Here we have tested that prediction in adherent human airway smooth muscle (HASM) cells. Traction microscopy was used to measure the distribution of contractile stresses arising at the interface between each cell and its substrate; this distribution is called the traction field. Because the traction field must be balanced by tensile stresses within the cell body, the prestress could be computed. Cell stiffness (G) was measured by oscillatory magnetic twisting cytometry. As the contractile state of the cell was modulated with graded concentrations of relaxing or contracting agonists (isoproterenol or histamine, respectively), the mean prestress ( t ) ranged from 350 to 1,900 Pa. Over that range, cell stiffness increased linearly with the prestress: G (Pa) = 0.18 t + 92. While this association does not necessarily preclude other interpretations, it is the hallmark of systems that secure shape stability mainly through the prestress. Regardless of mechanism, these data establish a strong association between stiffness of HASM cells and the level of tensile stress within the cytoskeleton.
Keywords
This publication has 56 references indexed in Scilit:
- The Role of Prestress and Architecture of the Cytoskeleton and Deformability of Cytoskeletal Filaments in Mechanics of Adherent Cells: a Quantitative AnalysisJournal of Theoretical Biology, 1999
- A Tensegrity Structure With Buckling Compression Elements: Application to Cell MechanicsJournal of Applied Mechanics, 1997
- Balanced mechanical forces and microtubule contribution to fibroblast contractionJournal of Cellular Physiology, 1996
- A Microstructural Approach to Cytoskeletal Mechanics based on TensegrityJournal of Theoretical Biology, 1996
- Probing transmembrane mechanical coupling and cytomechanics using magnetic twisting cytometryBiochemistry and Cell Biology, 1995
- Elementary Mechanics of the Endothelium of Blood VesselsJournal of Biomechanical Engineering, 1993
- Determination of Cellular Mechanical Properties by Cell Poking, With an Application to LeukocytesJournal of Biomechanical Engineering, 1990
- The Application of a Homogeneous Half-Space Model in the Analysis of Endothelial Cell Micropipette MeasurementsJournal of Biomechanical Engineering, 1988
- Role of cortical tension in fibroblast shape and movementCell Motility, 1987
- Mechanical properties of the protoplasm of the sea urchin eggExperimental Cell Research, 1969