Controlled human wood smoke exposure: oxidative stress, inflammation and microvascular function
Open Access
- 27 March 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in Particle and Fibre Toxicology
- Vol. 9 (1), 7
- https://doi.org/10.1186/1743-8977-9-7
Abstract
Background: Exposure to wood smoke is associated with respiratory symptoms, whereas knowledge on systemic effects is limited. We investigated effects on systemic inflammation, oxidative stress and microvascular function (MVF) after controlled wood smoke exposure. Methods: In a randomised, double-blinded, cross-over study 20 non-smoking atopic subjects were exposed at rest to 14, 220, or 354 μg/m3 of particles from a well-burning modern wood stove for 3 h in a climate controlled chamber with 2 week intervals. We investigated the level of oxidatively damaged DNA, inflammatory markers and adhesion molecules before and 0, 6 and 20 h after exposure. Six h after exposure we measured MVF non-invasively by digital peripheral artery tonometry following arm ischemia. Results: The MVF score was unaltered after inhalation of clean air (1.58 ± 0.07; mean ± SEM), low (1.51 ± 0.07) or high (1.61 ± 0.09) concentrations of wood smoke particles in atopic subjects, whereas unexposed non-atopic subjects had higher score (1.91 ± 0.09). The level of oxidatively damaged DNA, mRNA of ITGAL, CCL2, TNF, IL6, IL8, HMOX1, and OGG1 and surface marker molecules ICAM1, ITGAL and L-selectin in peripheral blood mononuclear cells were not affected by inhalation of wood smoke particles. Conclusions: Exposure to wood smoke had no effect on markers of oxidative stress, DNA damage, cell adhesion, cytokines or MVF in atopic subjects.Keywords
This publication has 51 references indexed in Scilit:
- Combustion-derived nanoparticulate induces the adverse vascular effects of diesel exhaust inhalationEuropean Heart Journal, 2011
- Assessment of Vascular Endothelial Function With Peripheral Arterial TonometryCardiology in Review, 2010
- An ECVAG trial on assessment of oxidative damage to DNA measured by the comet assayMutagenesis, 2009
- Oxidatively damaged DNA and its repair after experimental exposure to wood smoke in healthy humansMutation Research - Reviews in Mutation Research, 2008
- Variation in assessment of oxidatively damaged DNA in mononuclear blood cells by the comet assay with visual scoringMutagenesis, 2008
- Indoor Particles Affect Vascular Function in the AgedAmerican Journal of Respiratory and Critical Care Medicine, 2008
- Deposition of Biomass Combustion Aerosol Particles in the Human Respiratory TractInhalation Toxicology, 2008
- Exposure to ultrafine particles from ambient air and oxidative stress-induced DNA damageEnvironmental Health Perspectives, 2007
- Experimental Wood Smoke Exposure in HumansInhalation Toxicology, 2006
- Effects of ambient air pollution on symptoms of asthma in Seattle-area children enrolled in the CAMP study.Environmental Health Perspectives, 2000