The Cotton Rat as an Experimental Model of Human Parainfluenza Virus Type 3 Disease
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis Ltd in Experimental Lung Research
- Vol. 2 (2), 97-109
- https://doi.org/10.3109/01902148109052306
Abstract
Intranasal or aerosol inoculation of cotton rats (Sigmodon hispidus) with human parainfluenza virus type 3 (P3) produces pulmonary changes which closely resemble human disease due to this agent. Active viral replication resulted in peak titers by day 2 of infection. Viral antigen was localized by immunofluorescence in bronchial epithelial cells alone (aerosol inoculation) or epithelial and alveolar cells (intranasal instillation). Cytopathic effects in epithelial cells was noted as early as day 2, and marked cellular proliferation occurred by day 7. Intranasal instillation of virus resulted in development of patchy interstitial pneumonitis. Mononuclear cell infiltration in the peribronchial and perivascular areas peaked on days 6 and 7. A brisk systemic antibody response was measured both by complement fixation and virus neutralization techniques. These data establish the value of the cotton rat as an experimental model for the study of bronchiolitis and pneumonia due to P3. Since previous work has demonstrated that respiratory syncytial virus and Mycoplasma pneumoniae also will replicate and cause pulmonary disease in cotton rats, comparative studies in a single animal species are facilitated.Keywords
This publication has 8 references indexed in Scilit:
- Experimental infection of the respiratory tract with Mycoplasma pneumoniae.Environmental Health Perspectives, 1980
- Pulmonary distribution of particles given by intratracheal instillation or by aerosol inhalationEnvironmental Research, 1976
- Parainfluenza virus infectionsPreventive Medicine, 1974
- Pathological changes in virus infections of the lower respiratory tract in childrenJournal of Clinical Pathology, 1970
- EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT)The Journal of Experimental Medicine, 1965
- Parainfluenza Type 3 Virus Infection in Hamsters: Virologic, Serologic, and Pathologic StudiesThe Journal of Infectious Diseases, 1964
- Infection of Hamsters with Para Influenza 3 VirusExperimental Biology and Medicine, 1960
- An Infectious Agent from Cases of Atypical Pneumonia Apparently Transmissible to Cotton RatsScience, 1942